Bio-101 - Improve Research Reproducibility

Isolation of PDIM

Authors: CJ Cambier and Carolyn R Bertozzi
Date: Apr 11, 2023，Views：201

Original protocol used in research article：

Spreading of a mycobacterial cell-surface lipid into host epithelial membranes promotes infectivity
eLife, Nov 23, 2020; DOI: 10.7554/eLife.60648

Bacterial quantification

Authors: Christopher E. Pope and Tamara D. Simon
Date: Apr 11, 2023，Views：191

Original protocol used in research article：

Characterization of cerebrospinal fluid (CSF) microbiota from patients with CSF shunt infection and reinfection using high throughput sequencing of 16S ribosomal RNAgenes
PLoS ONE, Jan 6, 2021; DOI: 10.1371/journal.pone.0244643

Akinete Differentiation and Germination

Authors: Ritu Garg, Iris Maldener and Rebeca Perez
Date: Apr 11, 2023，Views：159

Original protocol used in research article：

The Dual Role of the Glycolipid Envelope in Different Cell Types of the Multicellular Cyanobacterium Anabaena variabilis ATCC 29413
Frontiers in Microbiology, Apr 9, 2021; DOI: 10.3389/fmicb.2021.645028

In vitro TFH stimulation assay

Authors: Phillip P. Domeier, Ziaur S.M. Rahman and Steven F. Ziegler
Date: Apr 10, 2023，Views：202

Original protocol used in research article：

B cell– and T cell–intrinsic regulation of germinal centers by thymic stromal lymphopoietin signaling
Science Immunology, Jan 6, 2023; DOI: 10.1126/sciimmunol.add9413

Genome-wide CRISPR-Cas9 screen

Authors: Zengzhang Zheng, Judy Lieberman, Xing Liu and Zengzhang Zheng
Date: Apr 6, 2023，Views：263

Original protocol used in research article：

The lysosomal Rag-Ragulator complex licenses RIPK1– and caspase-8–mediated pyroptosis by Yersinia
Science, Jun 25, 2021; DOI: 10.1126/science.abg0269

Organoid infection

Authors: Guillaume Jacquemin, Silvia Fre and Mathilde Huyghe
Date: Apr 6, 2023，Views：244

Original protocol used in research article：

Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme
eLife, ; DOI: 10.7554/eLife.76541

Generation of stable cell lines expressing 3xFLAG-CDK12

Authors: Xiangbing Qi and Ting Han
Date: Apr 5, 2023，Views：239

Original protocol used in research article：

Discovery of a molecular glue promoting CDK12-DDB1 interaction to trigger cyclin K degradation
eLife, Aug 17, 2020; DOI: 10.7554/eLife.59994

Cell cluster annotation, trajectory inference, and signature enrichment

Authors: Ekaterini Maria Lyras and Alexander Mildner
Date: Apr 5, 2023，Views：289

Original protocol used in research article：

Tongue immune compartment analysis reveals spatial macrophage heterogeneity
eLife, ; DOI: 10.7554/eLife.77490

Expression and purification of CR3022 Fab and IgG

Authors: Meng Yuan, Nicholas C. Wu, Wenli Yu and Ian A. Wilson
Date: Apr 4, 2023，Views：51

Original protocol used in research article：

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV
Science, May 7, 2020; DOI: 10.1126/science.abb7269

Preparation of odorant and buffer solutions

Authors: Albert Lin, Cengiz Pehlevan, Mei Zhen and Aravinthan D.T. Samuel
Date: Apr 4, 2023，Views：308

Original protocol used in research article：

Functional imaging and quantification of multineuronal olfactory responses in C. elegans
Science Advances, Mar 1, 2023; DOI: 10.1126/sciadv.ade1249

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