Protocols shared by authors via RaP service
Journal
Myotube-to-macrophage conditioned medium
Authors: Nicolas J. Pillon and Juleen R. Zierath
Date: Mar 7, 2023，Views：359
Original protocol used in research article：
Distinctive exercise-induced inflammatory response and exerkine induction in skeletal muscle of people with type 2 diabetes
Science Advances, Sep 7, 2022; DOI: 10.1126/sciadv.abo3192
DNA pull-down assays
Author: Matthew W Parker
Date: Mar 6, 2023，Views：460
Original protocol used in research article：
A new class of disordered elements controls DNA replication through initiator self-assembly
eLife, Sep 27, 2019; DOI: 10.7554/eLife.48562
LipidTOX staining
Authors: Meredith H Wilson and Steven A Farber
Date: Mar 6, 2023，Views：451
Original protocol used in research article：
Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
eLife, Aug 13, 2021; DOI: 10.7554/eLife.66393
Hepatic triglyceride content
Authors: Eric Chang, K. Singer and Mita Varghese
Date: Mar 6, 2023，Views：373
Original protocol used in research article：
Programming effects of maternal and gestational obesity on offspring metabolism and metabolic inflammation
Scientific Reports, Nov 5, 2019; DOI: 10.1038/s41598-019-52583-x
DNA fiber assays
Authors: Sharon B. Cantor and Sumeet Nayak
Date: Mar 4, 2023，Views：475
Original protocol used in research article：
Inhibition of the translesion synthesis polymerase REV1 exploits replication gaps as a cancer vulnerability
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz7808
Preparation of fibrin gel components
Authors: Ugur Uslu and Carl H. June
Date: Mar 4, 2023，Views：414
Original protocol used in research article：
Chimeric antigen receptor T cells as adjuvant therapy for unresectable adenocarcinoma
Science Advances, Jan 11, 2023; DOI: 10.1126/sciadv.ade2526
Candidate-gene association mapping
Authors: Xuan Zhang, Jiansheng Li, Jianbing Yan and Xiaohong Yang
Date: Mar 2, 2023，Views：393
Original protocol used in research article：
Convergent selection of a WD40 protein that enhances grain yield in maize and rice
Science, Mar 25, 2022; DOI: 10.1126/science.abg7985
Recombinant protein purification and pulldown
Authors: Gunther Hollopeter, Erik M Jorgensen and Gwendolyn Marie Beacham
Date: Mar 2, 2023，Views：396
Original protocol used in research article：
The membrane-associated proteins FCHo and SGIP are allosteric activators of the AP2 clathrin adaptor complex
eLife, Oct 10, 2014; DOI: 10.7554/eLife.03648
Perfusion and section preparation
Authors: Zheng Gan and Rohini Kuner
Date: Mar 2, 2023，Views：379
Original protocol used in research article：
Layer-specific pain relief pathways originating from primary motor cortex
Science, Dec 22, 2022; DOI: 10.1126/science.add4391
Nitro-blue tetrazolium (NBT) assay
Author: Thora Lieke
Date: Mar 1, 2023，Views：499
Original protocol used in research article：
Phenol-rich fulvic acid as a water additive enhances growth, reduces stress, and stimulates the immune system of fish in aquaculture
Scientific Reports, Jan 8, 2021; DOI: 10.1038/s41598-020-80449-0
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