Protocols shared by authors via RaP service
Journal
Experimental model and subject details
Author: Lucas Pozzo-Miller
Date: Apr 4, 2023，Views：26
Original protocol used in research article：
Ventral hippocampal projections to the medial prefrontal cortex regulate social memory
eLife, May 21, 2019; DOI: 10.7554/eLife.44182
Detection of mitochondrial ROS levels using flow cytometry
Authors: Xiaowei Zheng and Sergiu-Bogdan Catrina
Date: Apr 3, 2023，Views：370
Original protocol used in research article：
Repression of hypoxia-inducible factor-1 contributes to increased mitochondrial reactive oxygen species production in diabetes
eLife, Feb 15, 2022; DOI: 10.7554/eLife.70714
Generation of rescued mouse ES cell lines expressing EGFP-tagged Suv39h proteins
Authors: Thomas Jenuwein and Kalina Malgorzata Swist-Rosowska
Date: Mar 31, 2023，Views：264
Original protocol used in research article：
Major satellite repeat RNA stabilize heterochromatin retention of Suv39h enzymes by RNA-nucleosome association and RNA:DNA hybrid formation
eLife, Aug 1, 2017; DOI: 10.7554/eLife.25293
Reagents and infection with SARS-CoV-2
Authors: Gustavo Gastão Davanzo and Marcelo Hill
Date: Mar 27, 2023，Views：250
Original protocol used in research article：
PD-1/PD-L1 blockade abrogates a dysfunctional innate-adaptive immune axis in critical β-coronavirus disease
Science Advances, Sep 21, 2022; DOI: 10.1126/sciadv.abn6545
Immunofluorescence
Author: Sarah E Siegrist
Date: Mar 27, 2023，Views：270
Original protocol used in research article：
Eyeless uncouples mushroom body neuroblast proliferation from dietary amino acids in Drosophila
eLife, Aug 9, 2017; DOI: 10.7554/eLife.26343
Widefield and two-photon calcium imaging
Author: Matthew McGill
Date: Mar 27, 2023，Views：281
Original protocol used in research article：
Neural signatures of auditory hypersensitivity following acoustic trauma
eLife, Sep 16, 2022; DOI: 10.7554/eLife.80015
Adapted ATAC-seq protocol from Monahan et al eLife 2017
Author: Kevin Gunther Monahan
Date: Mar 27, 2023，Views：293
Original protocol used in research article：
Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
eLife, Sep 21, 2017; DOI: 10.7554/eLife.28620
Human-induced pluripotent stem cell culture
Author: Jan M Bruder
Date: Mar 27, 2023，Views：50
Original protocol used in research article：
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids
eLife, Nov 3, 2020; DOI: 10.7554/eLife.52904
Chlorophyllase activity assay
Authors: Sitian Gu and Yuanfa Liu
Date: Mar 26, 2023，Views：261
Original protocol used in research article：
Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304
Microbial Cell Factories, Jan 12, 2021; DOI: 10.1186/s12934-020-01507-w
Data processing on the rod-shaped WSSV capsids
Authors: Yu-Zhong Zhang and Qing-Tao Shen
Date: Mar 19, 2023，Views：14
Original protocol used in research article：
Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
Science Advances, Feb 22, 2023; DOI: 10.1126/sciadv.add2796
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