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CUT&RUN library preparation

PF Paolo Emanuele Forni

Last updated date: Jun 20, 2023 Views: 37 Forks: 0

original An abbreviated version of this protocol was published in eLife
Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons
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Fragment analysis of prepared CUT&RUN libraries was performed as a fee-for-service via Cornell University’s Genomics Core facility. DNA library quality was assessed by qualitatively visualizing fragment traces for fragments of the expected size (200-1000bp). Traces displaying empty adaptors (130bp) or free floating primers (65bp) were subjected to an additional SPRI bead cleanup to exclude unwanted fragments.

Copyright: © 2023 The Author(s); This is an open-access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Forni, P E(2023). CUT&RUN library preparation. Bio-protocol Preprint. bio-protocol.org/prep2341.
  2. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. eLife. DOI: 10.7554/eLife.77259

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