Bio-101 - Improve Research Reproducibility

Protein purification, MST, and EMSAs

Authors: Shengze Yao and Yi Li
Date: May 23, 2023，Views：754

Original protocol used in research article：

The key micronutrient copper orchestrates broad-spectrum virus resistance in rice
Science Advances, Jul 1, 2022; DOI: 10.1126/sciadv.abm0660

Purification of DNA Origami Nanostructures

Authors: Pierre Stömmer and Hendrik Dietz
Date: May 23, 2023，Views：733

Original protocol used in research article：

Cryo-Electron Microscopy and Mass Analysis of Oligolysine-Coated DNA Nanostructures
ACS Nano, Mar 16, 2021; DOI: 10.1021/acsnano.0c10137

Data acquisition of resting-state fMRI and optogenetic fMRI experiments

Authors: Jiaming Liu, Ed X. Wu, Yanqiu Feng and Xiong Cao
Date: May 23, 2023，Views：636

Original protocol used in research article：

Astrocyte dysfunction drives abnormal resting-state functional connectivity in depression
Science Advances, Nov 16, 2022; DOI: 10.1126/sciadv.abo2098

Macrophage depletion and reconstitution

Authors: Kang Yi Su and Wei-Jia Luo
Date: May 23, 2023，Views：590

Original protocol used in research article：

HLJ1 amplifies endotoxin-induced sepsis severity by promoting IL-12 heterodimerization in macrophages
eLife, ; DOI: 10.7554/eLife.76094

PLZF knockdown using siRNA

Author: Shuchi Agrawal Singh
Date: May 22, 2023，Views：568

Original protocol used in research article：

PLZF targets developmental enhancers for activation during osteogenic differentiation of human mesenchymal stem cells
eLife, Jan 23, 2019; DOI: 10.7554/eLife.40364

Generation of nanobody dimers by sortase-mediated functionalization and click chemistry

Author: Leo Hanke
Date: May 22, 2023，Views：568

Original protocol used in research article：

Multivariate mining of an alpaca immune repertoire identifies potent cross-neutralizing SARS-CoV-2 nanobodies
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm0220

Differentiation of rTTA/Ngn2-positive PSCs to iNeurons

Authors: Maria Schörnig, Valentina Rava and Elena Taverna
Date: May 22, 2023，Views：651

Original protocol used in research article：

Comparison of induced neurons reveals slower structural and functional maturation in humans than in apes
eLife, Jan 20, 2021; DOI: 10.7554/eLife.59323

Sequences (Figs 1-3)

Authors: Morgane Rolland and Eric Lewitus
Date: May 17, 2023，Views：31

Original protocol used in research article：

Design of a pan-betacoronavirus vaccine candidate through a phylogenetically informed approach
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.abq4149

EGFR Internalization and EGFR Recycling Assays

Authors: Chia-Hsing Shen and Li-Chung Hsu
Date: May 17, 2023，Views：685

Original protocol used in research article：

ZNRF1 Mediates Epidermal Growth Factor Receptor Ubiquitination to Control Receptor Lysosomal Trafficking and Degradation
Frontiers in Cell and Developmental Biology, Apr 29, 2021; DOI: 10.3389/fcell.2021.642625

Denaturing in vivo ubiquitination assay

Authors: Tyler B Faust, Amit Weiss, Bhargavi Jayaraman, Billy W Newton, Nevan J Krogan, Iván D'Orso and Alan D Frankel
Date: May 16, 2023，Views：768

Original protocol used in research article：

The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation
eLife, May 30, 2018; DOI: 10.7554/eLife.31879

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