Protocols shared by authors via RaP service
Journal
Tissue processing for single-cell isolation
Authors: Ana J Caetano and Paul Sharpe
Date: May 3, 2023，Views：499
Original protocol used in research article：
Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease
eLife, Jan 4, 2021; DOI: 10.7554/eLife.62810
Yeast agglutination assay
Authors: Sobieszczańska Beata and Dzięgiel Piotr
Date: May 2, 2023，Views：941
Original protocol used in research article：
Norepinephrine affects the interaction of adherent-invasive Escherichia coli with intestinal epithelial cells
Virulence, Feb 4, 2021; DOI: 10.1080/21505594.2021.1882780
Preparation of monolayers before mechanical strain
Author: Blair W Benham-Pyle
Date: May 1, 2023，Views：473
Original protocol used in research article：
Increasing β-catenin/Wnt3A activity levels drive mechanical strain-induced cell cycle progression through mitosis
eLife, Oct 26, 2016; DOI: 10.7554/eLife.19799
Fatty acid preparation
Authors: Sathish Kumar Natarajan and Ann Anderson-Berry
Date: May 1, 2023，Views：488
Original protocol used in research article：
Saturated free fatty acids induce placental trophoblast lipoapoptosis
PLoS ONE, Apr 22, 2021; DOI: 10.1371/journal.pone.0249907
Preparation of recombinant ITs
Author: Vidyalakshmi Chandramohan
Date: Apr 29, 2023，Views：432
Original protocol used in research article：
Immunotoxin-αCD40 therapy activates innate and adaptive immunity and generates a durable antitumor response in glioblastoma models
Science Translational Medicine, Feb 8, 2023; DOI: 10.1126/scitranslmed.abn5649
Transfection of miRNA Mimic or Inhibitor
Authors: Si Wang, Jing Xu, Yuanxu Guo, Yongsong Cai, Wenhua Zhu, Liesu Meng, Congshan Jiang and Shemin Lu
Date: Apr 29, 2023，Views：491
Original protocol used in research article：
MicroRNA-497 Reduction and Increase of Its Family Member MicroRNA-424 Lead to Dysregulation of Multiple Inflammation Related Genes in Synovial Fibroblasts With Rheumatoid Arthritis
Frontiers in Immunology, Mar 26, 2021; DOI: 10.3389/fimmu.2021.619392
Generation of transgenic Xist-BglG Halo ES cell line
Authors: Heather Coker, Lothar Schermelleh and Neil Brockdorff
Date: Apr 28, 2023，Views：435
Original protocol used in research article：
Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading
Science, Jun 11, 2021; DOI: 10.1126/science.abe7500
Purification and treatment of recombinant human RyR2
Authors: Marco C. Miotto and Andrew R. Marks
Date: Apr 27, 2023，Views：415
Original protocol used in research article：
Structural analyses of human ryanodine receptor type 2 channels reveal the mechanisms for sudden cardiac death and treatment
Science Advances, Jul 20, 2022; DOI: 10.1126/sciadv.abo1272
DNA fibre assay
Authors: Kasper Fugger and Stephen C. West
Date: Apr 27, 2023，Views：427
Original protocol used in research article：
Targeting the nucleotide salvage factor DNPH1 sensitizes BRCA-deficient cells to PARP inhibitors
Science, Apr 8, 2021; DOI: 10.1126/science.abb4542
4.8.1. BSA Denaturation InhibitionAssay
Authors: Rajlakshmi Devi and Devasish Chowdhury
Date: Apr 26, 2023，Views：843
Original protocol used in research article：
Green Synthesis of Gold Nano-bioconjugates from Onion Peel Extract and Evaluation of Their Antioxidant, Anti-inflammatory, and Cytotoxic Studies
ACS Omega, Jul 8, 2021; DOI: 10.1021/acsomega.1c00861
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