Protocols shared by authors via RaP service
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Biochemical measurements of ATP and ADP
Author: Masak Takaine
Date: May 11, 2023，Views：568
Original protocol used in research article：
High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast
eLife, Apr 19, 2022; DOI: 10.7554/eLife.67659
DGE analysis
Authors: Martin Jinye Zhang, Angela Oliveira Pisco and James Zou
Date: May 10, 2023，Views：446
Original protocol used in research article：
Mouse aging cell atlas analysis reveals global and cell type-specific aging signatures
eLife, Apr 13, 2021; DOI: 10.7554/eLife.62293
Interbacterial competition assay
Authors: Ethel Bayer-Santos and Daniel Enrique Sanchez-Limache
Date: May 10, 2023，Views：500
Original protocol used in research article：
Antibacterial T6SS effectors with a VRR-Nuc domain are structure-specific nucleases
eLife, Oct 13, 2022; DOI: 10.7554/eLife.82437
An optimized four-step genome assembly strategy
Authors: Shilin Tian and Huabin Zhao
Date: May 10, 2023，Views：474
Original protocol used in research article：
Comparative analyses of bat genomes identify distinct evolution of immunity in Old World fruit bats
Science Advances, May 5, 2023; DOI: 10.1126/sciadv.add0141
DEV analysis
Authors: Florian Grziwotz, Arndt Telschow and Chih-hao Hsieh
Date: May 10, 2023，Views：38
Original protocol used in research article：
Anticipating the occurrence and type of critical transitions
Science Advances, Jan 6, 2023; DOI: 10.1126/sciadv.abq4558
Signal processing and SNR calculation, as applied to chronic electrophysiology experiments
Authors: Alejandro Carnicer-Lombarte, George G. Malliaras and Damiano G. Barone
Date: May 9, 2023，Views：404
Original protocol used in research article：
Functional neurological restoration of amputated peripheral nerve using biohybrid regenerative bioelectronics
Science Advances, Mar 22, 2023; DOI: 10.1126/sciadv.add8162
Drug administration
Authors: Kaitlyn E. DeMeulenaere and Murali Prakriya
Date: May 8, 2023，Views：79
Original protocol used in research article：
Regulation of neuropathic pain by microglial Orai1 channels
Science Advances, Jan 27, 2023; DOI: 10.1126/sciadv.ade7002
MD simulations
Authors: Dario De Vecchis, Enrica Bordignon and Markus A. Seeger
Date: May 8, 2023，Views：40
Original protocol used in research article：
The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells
Science Advances, Oct 12, 2022; DOI: 10.1126/sciadv.abn6845
Creating Arabidopsis mutants with CRISPR/Cas9 by using the plasmid pHEE401E
Authors: Upendo Lupanga and Karin Schumacher
Date: May 8, 2023，Views：565
Original protocol used in research article：
The Arabidopsis V-ATPase is localized to the TGN/EE via a seed plant-specific motif
eLife, Nov 25, 2020; DOI: 10.7554/eLife.60568
Study design-Senolytic doses in human livers using proof-of-concept perfusion
Authors: Stuart J. Forbes and Sofia Ferreira-Gonzalez
Date: May 8, 2023，Views：429
Original protocol used in research article：
Senolytic treatment preserves biliary regenerative capacity lost through cellular senescence during cold storage
Science Translational Medicine, Dec 7, 2022; DOI: 10.1126/scitranslmed.abj4375
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