Protocols shared by authors via RaP service
Journal
Auxin-inducible degradation
Authors: Mike Boxem and Jorian J Sepers
Date: Mar 17, 2023，Views：39
Original protocol used in research article：
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
eLife, Dec 10, 2020; DOI: 10.7554/eLife.62067
Plasmids for transfection
Authors: João Vasco Ferreira and Paulo Pereira
Date: Mar 16, 2023，Views：17
Original protocol used in research article：
LAMP2A regulates the loading of proteins into exosomes
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm1140
Purification, freezing, and thawing of 3D7 P. falciparum-infected red blood cells and antibody-dependent cellular cytotoxicity (ADCC ) assay
Authors: Savannah N. Lewis, Prasanna Jagannathan and Savannah Nicole Lewis
Date: Mar 14, 2023，Views：371
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Isolation of peripheral blood mononuclear cells (PBMCs) from whole blood and thawing
Authors: Savannah N. Lewis and Prasanna Jagannathan
Date: Mar 14, 2023，Views：356
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Collection of human gonadal cells
Authors: Wolfram H. Gruhn, Walfred W.C. Tang and M. Azim Surani
Date: Mar 13, 2023，Views：394
Original protocol used in research article：
Epigenetic resetting in the human germ line entails histone modification remodeling
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.ade1257
Enzyme linked immunosorbant assay (ELISA)
Authors: Qiang Gao, Xiaoqin Ge, Jinxing Lu, Changgui Li, Xiangxi Wang, Weidong Yin, Yanjun Zhang and Chuan Qin
Date: Mar 10, 2023，Views：371
Original protocol used in research article：
Development of an inactivated vaccine candidate for SARS-CoV-2
Science, Jul 2, 2020; DOI: 10.1126/science.abc1932
LSK FACS-Sorting
Authors: Cristina Martínez Valiente, Cristian Garcia-Ruiz, Beatriz Rosón, Alessandro Liquori, Elisa González-Romero, Raúl Fernández-González, Isabel Gómez-Redondo, José Cervera, Alfonso Gutierrez Adan and Alejandra Sanjuan-Pla
Date: Mar 10, 2023，Views：362
Original protocol used in research article：
Aberrant Alternative Splicing in U2af1/Tet2 Double Mutant Mice Contributes to Major Hematological Phenotypes
International Journal of Molecular Sciences, Jun 28, 2021; DOI: 10.3390/ijms22136963
Western blotting analysis
Authors: Joseph Wayne M Fowler and William C Sessa
Date: Mar 9, 2023，Views：400
Original protocol used in research article：
Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cells
eLife, Aug 12, 2022; DOI: 10.7554/eLife.79529
Lentivirus infection of organoids for inducible knockdown and activation
Author: Emma L Rawlins
Date: Mar 8, 2023，Views：1082
Original protocol used in research article：
A functional genetic toolbox for human tissue-derived organoids
eLife, Oct 6, 2021; DOI: 10.7554/eLife.67886
Human specimens and cells
Authors: Liam J. O’Neil, Mariana J. Kaplan and Carmelo Carmona-Rivera
Date: Mar 7, 2023，Views：351
Original protocol used in research article：
Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis
Science Advances, Oct 27, 2020; DOI: 10.1126/sciadv.abd2688
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