Protocols shared by authors via RaP service
Journal
Widefield and two-photon calcium imaging
Author: Matthew McGill
Date: Mar 27, 2023，Views：316
Original protocol used in research article：
Neural signatures of auditory hypersensitivity following acoustic trauma
eLife, Sep 16, 2022; DOI: 10.7554/eLife.80015
Adapted ATAC-seq protocol from Monahan et al eLife 2017
Author: Kevin Gunther Monahan
Date: Mar 27, 2023，Views：354
Original protocol used in research article：
Cooperative interactions enable singular olfactory receptor expression in mouse olfactory neurons
eLife, Sep 21, 2017; DOI: 10.7554/eLife.28620
Human-induced pluripotent stem cell culture
Author: Jan M Bruder
Date: Mar 27, 2023，Views：99
Original protocol used in research article：
A fully automated high-throughput workflow for 3D-based chemical screening in human midbrain organoids
eLife, Nov 3, 2020; DOI: 10.7554/eLife.52904
Chlorophyllase activity assay
Authors: Sitian Gu and Yuanfa Liu
Date: Mar 26, 2023，Views：281
Original protocol used in research article：
Molecular, structural and biochemical characterization of a novel recombinant chlorophyllase from cyanobacterium Oscillatoria acuminata PCC 6304
Microbial Cell Factories, Jan 12, 2021; DOI: 10.1186/s12934-020-01507-w
Data processing on the rod-shaped WSSV capsids
Authors: Yu-Zhong Zhang and Qing-Tao Shen
Date: Mar 19, 2023，Views：28
Original protocol used in research article：
Ring-stacked capsids of white spot syndrome virus and structural transitions with genome ejection
Science Advances, Feb 22, 2023; DOI: 10.1126/sciadv.add2796
Auxin-inducible degradation
Authors: Mike Boxem and Jorian J Sepers
Date: Mar 17, 2023，Views：80
Original protocol used in research article：
Epidermal PAR-6 and PKC-3 are essential for larval development of C. elegans and organize non-centrosomal microtubules
eLife, Dec 10, 2020; DOI: 10.7554/eLife.62067
Plasmids for transfection
Authors: João Vasco Ferreira and Paulo Pereira
Date: Mar 16, 2023，Views：42
Original protocol used in research article：
LAMP2A regulates the loading of proteins into exosomes
Science Advances, Mar 25, 2022; DOI: 10.1126/sciadv.abm1140
Purification, freezing, and thawing of 3D7 P. falciparum-infected red blood cells and antibody-dependent cellular cytotoxicity (ADCC ) assay
Authors: Savannah N. Lewis, Prasanna Jagannathan and Savannah Nicole Lewis
Date: Mar 14, 2023，Views：399
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Isolation of peripheral blood mononuclear cells (PBMCs) from whole blood and thawing
Authors: Savannah N. Lewis and Prasanna Jagannathan
Date: Mar 14, 2023，Views：382
Original protocol used in research article：
Malaria-driven expansion of adaptive-like functional CD56-negative NK cells correlates with clinical immunity to malaria
Science Translational Medicine, Jan 25, 2023; DOI: 10.1126/scitranslmed.add9012
Collection of human gonadal cells
Authors: Wolfram H. Gruhn, Walfred W.C. Tang and M. Azim Surani
Date: Mar 13, 2023，Views：428
Original protocol used in research article：
Epigenetic resetting in the human germ line entails histone modification remodeling
Science Advances, Jan 18, 2023; DOI: 10.1126/sciadv.ade1257
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