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Last updated date: May 23, 2023 Views: 545 Forks: 0
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For macrophage depletion and reconstitution, we referred to the protocol published by Weisser, S.B. et al., (2012). Bone marrow-derived macrophage isolation protocol was modified from a paper published by Trouplin et al., (2013). For in vivo macrophage depletion, the liposome-encapsulated clodronate was administrated according to the manufacturer’s protocol. We wrote down our version of the combined protocol as detailed as possible and are happy to address questions about the procedure should there be anything unclear.
Author: Wei-Jia Luo, Ph.D. Email address: f04424006@ntu.edu.tw
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