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Proteomics and untargeted metabolomics from plasma

KA Kazuo Ando

Last updated date: Jul 17, 2023 Views: 907 Forks: 0

original An abbreviated version of this protocol was published in Science Translational Medicine in May, 2021
Integrated trajectories of the maternal metabolome, proteome, and immunome predict labor onset
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Participants will be consented to participate prior to study
interventions.

Blood samples (10ml each) will be drawn sequentially throughout
pregnancy beginning at 24 weeks at antenatal care visits throughout
the pregnancy. The goal is to obtain between 3 and 5 blood samples
before delivery. A final sample will be drawn at the 6 week
postpartum visit, or at a time and place convenient for the
participant. These samples will be analyzed in the Gaudilliere lab at
Stanford using CYTof technology. An interim analysis of these samples
will be conducted after 20 participants have completed the study to
optimize the analysis of the remaining 60 participants. Enrollment
will continue throughout this period.

In addition to blood samples we will collect basic demographic data,
obstetric and general health history information, delivery data for
the current pregnancy, and general information on the health of the
delivered infant.

Copyright: © 2023 The Author(s); This is an open-access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/).
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Ando, K(2023). Proteomics and untargeted metabolomics from plasma. Bio-protocol Preprint. bio-protocol.org/prep2373.
  2. Stelzer, I. A., Ghaemi, M. S., Han, X., Ando, K., Hédou, J. J., Feyaerts, D., Peterson, L. S., Rumer, K. K., Tsai, E. S., Ganio, E. A., Gaudillière, D. K., Tsai, A. S., Choisy, B., Gaigne, L. P., Verdonk, F., Jacobsen, D., Gavasso, S., Traber, G. M., Ellenberger, M., Stanley, N., Becker, M., Culos, A., Fallahzadeh, R., Wong, R. J., Darmstadt, G. L., Druzin, M. L., Winn, V. D., Gibbs, R. S., Ling, X. B., Sylvester, K., Carvalho, B., Snyder, M. P., Shaw, G. M., Stevenson, D. K., Contrepois, K., Angst, M. S., Aghaeepour, N. and Gaudillière, B.(2021). Integrated trajectories of the maternal metabolome, proteome, and immunome predict labor onset. Science Translational Medicine 13(592). DOI: 10.1126/scitranslmed.abd9898

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