Protocols shared by authors via RaP service
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Immunoprecipitation and GST pull-down
Author: Arnaud Besson
Date: Sep 29, 2020，Views：1078
Original protocol used in research article：
p27Kip1 promotes invadopodia turnover and invasion through the regulation of the PAK1/Cortactin pathway
eLife, Mar 13, 2017; DOI: 10.7554/eLife.22207
Measurement of heat production (thermogenesis) in cells using ERthermAC dye
Authors: Chih-Hao Wang and Yu-Hua Tseng
Date: Sep 29, 2020，Views：1583
Original protocol used in research article：
CRISPR-engineered human brown-like adipocytes prevent diet-induced obesity and ameliorate metabolic syndrome in mice
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aaz8664
Heparin-induced filament assembly of tau
Author: Wenjuan Zhang
Date: Sep 28, 2020，Views：1633
Original protocol used in research article：
Heparin-induced tau filaments are polymorphic and differ from those in Alzheimer’s and Pick’s diseases
eLife, Feb 5, 2019; DOI: 10.7554/eLife.43584
Flow cytometry
Authors: Christian Perez-Shibayama, Cristina Gil-Cruz and Burkhard Ludewig
Date: Sep 24, 2020，Views：947
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Droplet-based scRNA-seq analysis
Authors: Christian Perez-Shibayama, Mechthild Luedge and Burkhard Ludewig
Date: Sep 24, 2020，Views：878
Original protocol used in research article：
Type I interferon signaling in fibroblastic reticular cells prevents exhaustive activation of antiviral CD8+ T cells
Science Immunology, Sep 11, 2020; DOI: 10.1126/sciimmunol.abb7066
Colorimetric RT-LAMP and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Authors: Viet Loan Dao Thi, Michael Knop, Simon Anders, Konrad Herbst, Matthias Meurer and Daniel Kirrmaier
Date: Sep 24, 2020，Views：924
Original protocol used in research article：
A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples
Science Translational Medicine, Aug 12, 2020; DOI: 10.1126/scitranslmed.abc7075
Untargeted metabolomics
Author: Carlos Guijas
Date: Sep 23, 2020，Views：958
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
Stimulus Ordering
Authors: Michael S Beauchamp and Michael Beauchamp
Date: Sep 23, 2020，Views：910
Original protocol used in research article：
The visual speech head start improves perception and reduces superior temporal cortex responses to auditory speech
eLife, Aug 8, 2019; DOI: 10.7554/eLife.48116
In vitro cytotoxicity assay
Author: Kun Cheng
Date: Sep 22, 2020，Views：873
Original protocol used in research article：
Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb0616
Cellular uptake study
Author: Kun Cheng
Date: Sep 22, 2020，Views：867
Original protocol used in research article：
Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
Science Advances, Jul 14, 2020; DOI: 10.1126/sciadv.abb0616
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