Protocols shared by authors via RaP service
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Skin-penetrating IL-RNA complexes
Author: Abhirup Mandal
Date: Sep 18, 2020，Views：972
Original protocol used in research article：
Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.abb6049
Statistics
Author: Carlos Guijas
Date: Sep 18, 2020，Views：826
Original protocol used in research article：
Metabolic adaptation to calorie restriction
Science Signaling, Sep 8, 2020; DOI: 10.1126/scisignal.abb2490
Immunostaining of fish scales
Author: Valerie Wittamer
Date: Sep 18, 2020，Views：873
Original protocol used in research article：
Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes
eLife, May 5, 2020; DOI: 10.7554/eLife.53403
Immunofluorescence
Authors: Ruihua Ma and Beatriz Sosa-Pineda
Date: Sep 17, 2020，Views：999
Original protocol used in research article：
Metabolic and non-metabolic liver zonation is established non-synchronously and requires sinusoidal Wnts
eLife, Mar 10, 2020; DOI: 10.7554/eLife.46206
RNAscope in situ hybridization
Author: Glenda Comai
Date: Sep 16, 2020，Views：1799
Original protocol used in research article：
A distinct cardiopharyngeal mesoderm genetic hierarchy establishes antero-posterior patterning of esophagus striated muscle
eLife, Sep 19, 2019; DOI: 10.7554/eLife.47460
Flow cytometry antibody panel
Authors: Alexandre Boissonnas and Pierre Louis Loyher
Date: Sep 16, 2020，Views：957
Original protocol used in research article：
Immune surveillance of the lung by migrating tissue monocytes
eLife, Jul 13, 2015; DOI: 10.7554/eLife.07847
Cryo–electron tomography
Authors: M. A. Benhaim, K. K. Lee and Vidya Mangala Prasad
Date: Sep 15, 2020，Views：814
Original protocol used in research article：
Structural monitoring of a transient intermediate in the hemagglutinin fusion machinery on influenza virions
Science Advances, Apr 28, 2020; DOI: 10.1126/sciadv.aaz8822
Exosome isolation, Subcellular fractionation, and TCA precipitation
Author: Karoliina Stefanius
Date: Sep 14, 2020，Views：1092
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
Quantification of NaV1.1 Protein in Mouse Brains using Meso Scale Discovery (MSD) Method
Authors: Zhou Han and Gene Liau
Date: Sep 14, 2020，Views：1028
Original protocol used in research article：
Antisense oligonucleotides increase Scn1a expression and reduce seizures and SUDEP incidence in a mouse model of Dravet syndrome
Science Translational Medicine, Aug 26, 2020; DOI: 10.1126/scitranslmed.aaz6100
NK stimulation assay
Author: Sytse Jan Piersma
Date: Sep 14, 2020，Views：921
Original protocol used in research article：
A herpesvirus encoded Qa-1 mimic inhibits natural killer cell cytotoxicity through CD94/NKG2A receptor engagement
eLife, Dec 21, 2018; DOI: 10.7554/eLife.38667
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