Protocols shared by authors via RaP service
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Pathogen inoculation procedure
Author: Shouan Liu
Date: Oct 27, 2020，Views：1310
Original protocol used in research article：
Negative regulation of ABA signaling by WRKY33 is critical for Arabidopsis immunity towards Botrytis cinerea 2100
eLife, Jun 15, 2015; DOI: 10.7554/eLife.07295
Purification
Author: Jia-Yin Tsai
Date: Oct 27, 2020，Views：822
Original protocol used in research article：
Structure of the sodium-dependent phosphate transporter reveals insights into human solute carrier SLC20
Science Advances, Aug 6, 2020; DOI: 10.1126/sciadv.abb4024
Imaging of microdroplets and image analysis
Author: SangMoon Lhee
Date: Oct 27, 2020，Views：788
Original protocol used in research article：
Spatial localization of charged molecules by salt ions in oil-confined water microdroplets
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.aba0181
Neoangiogenesis
Authors: D. A. Golombek and Natalia Paladino
Date: Oct 27, 2020，Views：859
Original protocol used in research article：
Circadian disruption promotes tumor-immune microenvironment remodeling favoring tumor cell proliferation
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.aaz4530
Fission yeast strains and general techniques
Authors: Tomoyuki Fukuda and Kazuhiro Shiozaki
Date: Oct 27, 2020，Views：808
Original protocol used in research article：
Ragulator and GATOR1 complexes promote fission yeast growth by attenuating TOR complex 1 through Rag GTPases
eLife, Dec 4, 2017; DOI: 10.7554/eLife.30880
Tissue preparation
Author: Pamela Maher
Date: Oct 27, 2020，Views：1027
Original protocol used in research article：
Elevating acetyl-CoA levels reduces aspects of brain aging
eLife, Nov 19, 2019; DOI: 10.7554/eLife.47866
Immunofluorescence and immunohistochemistry
Author: Emanuele Puca
Date: Oct 27, 2020，Views：814
Original protocol used in research article：
Immunocytokines are a promising immunotherapeutic approach against glioblastoma
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2311
Genetic manipulation of S. aureus mutants
Authors: Katarzyna Wacnik and Simon J Foster
Date: Oct 27, 2020，Views：2333
Original protocol used in research article：
Molecular coordination of Staphylococcus aureus cell division
eLife, Feb 21, 2018; DOI: 10.7554/eLife.32057
LC3 lipidation, membrane fractionation, immunodepletion, and immunoblot
Author: Liang Ge
Date: Oct 26, 2020，Views：1042
Original protocol used in research article：
Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-Golgi intermediate compartment
eLife, Nov 28, 2014; DOI: 10.7554/eLife.04135
Animal care and stress paradigm
Author: Jaideep Singh Bains
Date: Oct 26, 2020，Views：1129
Original protocol used in research article：
Sexually dimorphic neuronal responses to social isolation
eLife, Oct 11, 2016; DOI: 10.7554/eLife.18726
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