Protocols shared by authors via RaP service
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Primers information for quantitative PCR
Authors: Xiao Ma and Xiaolei Xu
Date: Dec 1, 2020，Views：1066
Original protocol used in research article：
Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aay2939
Fecal microbiota 16S rRNA gene sequencing
Author: Jakob Stokholm
Date: Dec 1, 2020，Views：869
Original protocol used in research article：
Delivery mode and gut microbial changes correlate with an increased risk of childhood asthma
Science Translational Medicine, Nov 10, 2020; DOI: 10.1126/scitranslmed.aax9929
Marble burying test
Author: Huda Y Zoghbi
Date: Nov 30, 2020，Views：1284
Original protocol used in research article：
Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome
eLife, Jun 21, 2016; DOI: 10.7554/eLife.14198
NMR spectroscopy and structure calculations
Author: Edward K. Gilding
Date: Nov 30, 2020，Views：854
Original protocol used in research article：
Neurotoxic peptides from the venom of the giant Australian stinging tree
Science Advances, Sep 16, 2020; DOI: 10.1126/sciadv.abb8828
Analysis of Ca2+ transients
Author: Katsutoshi Mizuno
Date: Nov 30, 2020，Views：915
Original protocol used in research article：
Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195
Enzymatic dispersal of Cerebral artery SMCs
Author: Scott Earley
Date: Nov 30, 2020，Views：861
Original protocol used in research article：
TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells
Science Signaling, Jun 22, 2020; DOI: 10.1126/scisignal.aba1015
Human NP cell isolation and culture
Author: Yongxin Ren
Date: Nov 30, 2020，Views：1084
Original protocol used in research article：
p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52570
Single cell ATAC-seq
Authors: Enric Llorens-Bobadilla and Jonas Frisén
Date: Nov 29, 2020，Views：971
Original protocol used in research article：
A latent lineage potential in resident neural stem cells enables spinal cord repair
Science, Oct 1, 2020; DOI: 10.1126/science.abb8795
Proliferation assay in vivo
Authors: Kishor K Sivaraj and Ralf H Adams
Date: Nov 27, 2020，Views：1100
Original protocol used in research article：
YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
eLife, Jan 20, 2020; DOI: 10.7554/eLife.50770
FIB-SEM dataset
Author: Kazunori Shinomiya
Date: Nov 25, 2020，Views：1126
Original protocol used in research article：
Comparisons between the ON- and OFF-edge motion pathways in the Drosophila brain
eLife, Jan 9, 2019; DOI: 10.7554/eLife.40025
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