Protocols shared by authors via RaP service
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Western blot analysis of cultured fibroblasts
Author: Philip S. Low
Date: Nov 22, 2020，Views：821
Original protocol used in research article：
Targeted inhibition of PI3 kinase/mTOR specifically in fibrotic lung fibroblasts suppresses pulmonary fibrosis in experimental models
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.aay3724
Recombinant IRF5 dimerization assay - protein preparation
Author: Gang Chen
Date: Nov 20, 2020，Views：1138
Original protocol used in research article：
Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057
Calculating scores and classifications
Author: Melissa Chiasson
Date: Nov 20, 2020，Views：852
Original protocol used in research article：
Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact
eLife, Sep 1, 2020; DOI: 10.7554/eLife.58026
Specificity scores
Authors: Silvia Domcke and Andrew J. Hill
Date: Nov 20, 2020，Views：951
Original protocol used in research article：
A human cell atlas of fetal chromatin accessibility
Science, Nov 13, 2020; DOI: 10.1126/science.aba7612
SARS-CoV-2 neutralization assay
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：966
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
LION formulation of the repRNA vaccine
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：937
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
Computational prediction of RNA structures
Author: Yan Zhang
Date: Nov 19, 2020，Views：969
Original protocol used in research article：
Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037
Promoter Sequences
Author: Tarik F. Haydar
Date: Nov 19, 2020，Views：947
Original protocol used in research article：
Transcriptional priming as a conserved mechanism of lineage diversification in the developing mouse and human neocortex
Science Advances, Nov 5, 2020; DOI: 10.1126/sciadv.abd2068
Single-molecule analysis of replication
Author: W. Liu
Date: Nov 19, 2020，Views：1036
Original protocol used in research article：
Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors
Science Advances, Nov 12, 2020; DOI: 10.1126/sciadv.abc3598
Phase separation using an optoDroplet system
Author: Yujie Sun
Date: Nov 18, 2020，Views：1029
Original protocol used in research article：
Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering
Science Advances, Apr 14, 2020; DOI: 10.1126/sciadv.aay6515
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