Protocols shared by authors via RaP service
Journal
Illumina sequencing and SNP calling
Author: Qingyun Liu
Date: Jan 7, 2021，Views：935
Original protocol used in research article：
Mycobacterium tuberculosis clinical isolates carry mutational signatures of host immune environments
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba4901
PG9 chimeric light chain IgG expression
Author: Roberta P Fuller
Date: Jan 7, 2021，Views：1170
Original protocol used in research article：
Reprogramming the antigen specificity of B cells using genome-editing technologies
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42995
Measurement of CD8+ T cell cytotoxicity
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Jan 6, 2021，Views：986
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Haemocytometry
Authors: Joachim Linssen and Andre J van der Ven
Date: Jan 5, 2021，Views：1224
Original protocol used in research article：
A novel haemocytometric COVID-19 prognostic score developed and validated in an observational multicentre European hospital-based study
eLife, Nov 26, 2020; DOI: 10.7554/eLife.63195
Generation of FAM150A-conditioned and FAM150B-conditioned media
Author: Jikui Guan
Date: Jan 5, 2021，Views：1034
Original protocol used in research article：
FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase
eLife, Sep 29, 2015; DOI: 10.7554/eLife.09811
Shelf life testing of antibody-functionalized microchips
Author: Hadi Shafiee
Date: Jan 4, 2021，Views：1022
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
In silico SETD2 methylation site analysis
Authors: Riyad N. H. Seervai and Cheryl Lyn Walker
Date: Jan 1, 2021，Views：948
Original protocol used in research article：
The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abb7854
Electromagnetic simulation
Authors: Jahyun Koo and Xie Zhaoqian
Date: Jan 1, 2021，Views：909
Original protocol used in research article：
Wirelessly controlled bioresorbable drug delivery device with active valves that exploit electrochemically triggered crevice corrosion
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.abb1093
Differential analysis of N-glycopeptide abundance and N-glycosylation site occupancy for studying protein N-glycosylation dysregulation in human disease
Authors: Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin
Date: Dec 30, 2020，Views：1179
Original protocol used in research article：
Integrative glycoproteomics reveals protein N-glycosylation aberrations and glycoproteomic network alterations in Alzheimer’s disease
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abc5802
RNA-seq expression analysis
Authors: Stefan Bohn and Nevan J. Krogan
Date: Dec 30, 2020，Views：1491
Original protocol used in research article：
Genetic interaction mapping informs integrative structure determination of protein complexes
Science, Dec 11, 2020; DOI: 10.1126/science.aaz4910
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