Protocols shared by authors via RaP service
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Generation of droplet-based artificial cells
Authors: Ye Guan, Shiyuan Wang and Qiong Yang
Date: Nov 2, 2020，Views：956
Original protocol used in research article：
A robust and tunable mitotic oscillator in artificial cells
eLife, Apr 5, 2018; DOI: 10.7554/eLife.33549
Golgi-cox staining, dendritic spine analysis and neuronal morphology
Author: W Christopher Risher
Date: Nov 2, 2020，Views：985
Original protocol used in research article：
Astrocytes refine cortical connectivity at dendritic spines
eLife, Dec 17, 2014; DOI: 10.7554/eLife.04047
In vitro efferocytosis
Authors: Arif Yurdagul Jr. and Ira Tabas
Date: Oct 31, 2020，Views：956
Original protocol used in research article：
siRNA nanoparticles targeting CaMKIIγ in lesional macrophages improve atherosclerotic plaque stability in mice
Science Translational Medicine, Jul 21, 2020; DOI: 10.1126/scitranslmed.aay1063
Statistical analysis
Authors: Cassondra L. Vernier and Yehuda Ben-Shahar
Date: Oct 30, 2020，Views：815
Original protocol used in research article：
The gut microbiome defines social group membership in honey bee colonies
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3431
Polysome and Puromycin immunoprecipitation
Authors: Benjamin D Hobson and Peter A Sims
Date: Oct 30, 2020，Views：1571
Original protocol used in research article：
Elongation inhibitors do not prevent the release of puromycylated nascent polypeptide chains from ribosomes
eLife, Aug 26, 2020; DOI: 10.7554/eLife.60048
Cell cycle prediction
Authors: Anneke Dixie Kakebeen and Andrea Elizabeth Wills
Date: Oct 29, 2020，Views：980
Original protocol used in research article：
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648
3D vascular imaging
Authors: Célian Bimbard, Charlie Demene and Yves Boubenec
Date: Oct 29, 2020，Views：856
Original protocol used in research article：
Multi-scale mapping along the auditory hierarchy using high-resolution functional UltraSound in the awake ferret
eLife, Jun 28, 2018; DOI: 10.7554/eLife.35028
List of antibodies used for cell sorting
Author: Jaromír Mikeš
Date: Oct 29, 2020，Views：807
Original protocol used in research article：
A genome-wide transcriptomic analysis of protein-coding genes in human blood cells
Science, Dec 19, 2019; DOI: 10.1126/science.aax9198
Compound treatment assay for C. elegans
Authors: Di Zhu and Ye Tian
Date: Oct 28, 2020，Views：868
Original protocol used in research article：
NuRD mediates mitochondrial stress–induced longevity via chromatin remodeling in response to acetyl-CoA level
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb2529
In vitro collagenase activity assay
Authors: Honoo Satake and Shin Matsubara
Date: Oct 28, 2020，Views：1308
Original protocol used in research article：
The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates
eLife, Oct 1, 2019; DOI: 10.7554/eLife.49062
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