Protocols shared by authors via RaP service
Journal
EC50-Determination of RSL3 towards bloodstream T. brucei
Authors: Marta Bogacz and R Luise Krauth-Siegel
Date: Nov 25, 2020，Views：983
Original protocol used in research article：
Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death
eLife, Jul 26, 2018; DOI: 10.7554/eLife.37503
Bioinformatic Analysis
Authors: Suzanne Rohrback, Sarah Munchel and Fiona Kaper
Date: Nov 24, 2020，Views：1063
Original protocol used in research article：
Circulating transcripts in maternal blood reflect a molecular signature of early-onset preeclampsia
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaz0131
Yeast Micro-C assay
Author: Tsung-Han S. Hsieh
Date: Nov 24, 2020，Views：983
Original protocol used in research article：
Cohesin residency determines chromatin loop patterns
eLife, Nov 10, 2020; DOI: 10.7554/eLife.59889
Stimuli
Author: Marwa El Zein
Date: Nov 24, 2020，Views：1135
Original protocol used in research article：
Anxiety dissociates the adaptive functions of sensory and motor response enhancements to social threats
eLife, Dec 29, 2015; DOI: 10.7554/eLife.10274
Y-maze, modified and spontaneous alternation task version
Authors: Da Hee Jung and Byung Tae Choi
Date: Nov 23, 2020，Views：5288
Original protocol used in research article：
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359
WGCNA network analysis
Author: Kathleen Greenham
Date: Nov 23, 2020，Views：1161
Original protocol used in research article：
Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in Brassica rapa
eLife, Aug 18, 2017; DOI: 10.7554/eLife.29655
‘On-bead’ peptidisc reconstitution
Author: Franck Duong
Date: Nov 23, 2020，Views：1046
Original protocol used in research article：
The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
eLife, Aug 15, 2018; DOI: 10.7554/eLife.34085
Western blot analysis of cultured fibroblasts
Author: Philip S. Low
Date: Nov 22, 2020，Views：836
Original protocol used in research article：
Targeted inhibition of PI3 kinase/mTOR specifically in fibrotic lung fibroblasts suppresses pulmonary fibrosis in experimental models
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.aay3724
Recombinant IRF5 dimerization assay - protein preparation
Author: Gang Chen
Date: Nov 20, 2020，Views：1153
Original protocol used in research article：
Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057
Calculating scores and classifications
Author: Melissa Chiasson
Date: Nov 20, 2020，Views：867
Original protocol used in research article：
Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact
eLife, Sep 1, 2020; DOI: 10.7554/eLife.58026
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