Protocols shared by authors via RaP service
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Enzymatic dispersal of Cerebral artery SMCs
Author: Scott Earley
Date: Nov 30, 2020，Views：832
Original protocol used in research article：
TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells
Science Signaling, Jun 22, 2020; DOI: 10.1126/scisignal.aba1015
Human NP cell isolation and culture
Author: Yongxin Ren
Date: Nov 30, 2020，Views：1016
Original protocol used in research article：
p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52570
Single cell ATAC-seq
Authors: Enric Llorens-Bobadilla and Jonas Frisén
Date: Nov 29, 2020，Views：936
Original protocol used in research article：
A latent lineage potential in resident neural stem cells enables spinal cord repair
Science, Oct 1, 2020; DOI: 10.1126/science.abb8795
Proliferation assay in vivo
Authors: Kishor K Sivaraj and Ralf H Adams
Date: Nov 27, 2020，Views：1064
Original protocol used in research article：
YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells
eLife, Jan 20, 2020; DOI: 10.7554/eLife.50770
FIB-SEM dataset
Author: Kazunori Shinomiya
Date: Nov 25, 2020，Views：1041
Original protocol used in research article：
Comparisons between the ON- and OFF-edge motion pathways in the Drosophila brain
eLife, Jan 9, 2019; DOI: 10.7554/eLife.40025
Fixed tissue preparation, immunohistochemistry, and imaging
Author: Adam J Granger
Date: Nov 25, 2020，Views：905
Original protocol used in research article：
Corelease of acetylcholine and GABA from cholinergic forebrain neurons
eLife, Feb 27, 2015; DOI: 10.7554/eLife.06412
EC50-Determination of RSL3 towards bloodstream T. brucei
Authors: Marta Bogacz and R Luise Krauth-Siegel
Date: Nov 25, 2020，Views：914
Original protocol used in research article：
Tryparedoxin peroxidase-deficiency commits trypanosomes to ferroptosis-type cell death
eLife, Jul 26, 2018; DOI: 10.7554/eLife.37503
Bioinformatic Analysis
Authors: Suzanne Rohrback, Sarah Munchel and Fiona Kaper
Date: Nov 24, 2020，Views：1004
Original protocol used in research article：
Circulating transcripts in maternal blood reflect a molecular signature of early-onset preeclampsia
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaz0131
Yeast Micro-C assay
Author: Tsung-Han S. Hsieh
Date: Nov 24, 2020，Views：942
Original protocol used in research article：
Cohesin residency determines chromatin loop patterns
eLife, Nov 10, 2020; DOI: 10.7554/eLife.59889
Stimuli
Author: Marwa El Zein
Date: Nov 24, 2020，Views：1092
Original protocol used in research article：
Anxiety dissociates the adaptive functions of sensory and motor response enhancements to social threats
eLife, Dec 29, 2015; DOI: 10.7554/eLife.10274
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