Protocols shared by authors via RaP service
Journal
Primers for Quantitative real-time PCR
Authors: Shunxing Rong and Jay D Horton
Date: Dec 4, 2020，Views：1147
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
Programming and reading schemes of the memristor-based system
Author: Jianshi Tang
Date: Dec 4, 2020，Views：888
Original protocol used in research article：
Multichannel parallel processing of neural signals in memristor arrays
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc4797
Acid-base titration
Author: Funeng Xu
Date: Dec 4, 2020，Views：889
Original protocol used in research article：
Development of a pH-responsive polymersome inducing endoplasmic reticulum stress and autophagy blockade
Science Advances, Jul 30, 2020; DOI: 10.1126/sciadv.abb8725
Staff screening protocols
Author: Nick K Jones
Date: Dec 3, 2020，Views：999
Original protocol used in research article：
Effective control of SARS-CoV-2 transmission between healthcare workers during a period of diminished community prevalence of COVID-19
eLife, Jun 19, 2020; DOI: 10.7554/eLife.59391
Sphere culture
Author: Tanya Das
Date: Dec 3, 2020，Views：881
Original protocol used in research article：
SMAR1 repression by pluripotency factors and consequent chemoresistance in breast cancer stem-like cells is reversed by aspirin
Science Signaling, Oct 19, 2020; DOI: 10.1126/scisignal.aay6077
CRISPR/Cas9-mediated editing and cell sorting
Authors: Li Fan and Honglin Wang
Date: Dec 2, 2020，Views：945
Original protocol used in research article：
A micropeptide encoded by lncRNA MIR155HG suppresses autoimmune inflammation via modulating antigen presentation
Science Advances, May 20, 2020; DOI: 10.1126/sciadv.aaz2059
Alternative poly-adenylation (APA) analysis
Authors: Hari Krishna Yalamanchili, Callison Edward Alcott, Zhandong Liu and Huda Yahya Zoghbi
Date: Dec 2, 2020，Views：839
Original protocol used in research article：
Partial loss of CFIm25 causes learning deficits and aberrant neuronal alternative polyadenylation
eLife, Apr 22, 2020; DOI: 10.7554/eLife.50895
Motor unit characteristics
Authors: A. Del Vecchio and D. Farina
Date: Dec 2, 2020，Views：784
Original protocol used in research article：
Spinal motoneurons of the human newborn are highly synchronized during leg movements
Science Advances, Nov 19, 2020; DOI: 10.1126/sciadv.abc3916
Preparation of single hemocytes in suspension
Authors: Sudhir Gopal Tattikota and Norbert Perrimon
Date: Dec 2, 2020，Views：1206
Original protocol used in research article：
A single-cell survey of Drosophila blood
eLife, May 12, 2020; DOI: 10.7554/eLife.54818
APEX2 reaction
Authors: Luis Bonet-Ponce and Mark R. Cookson
Date: Dec 2, 2020，Views：916
Original protocol used in research article：
LRRK2 mediates tubulation and vesicle sorting from lysosomes
Science Advances, Nov 11, 2020; DOI: 10.1126/sciadv.abb2454
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