Protocols shared by authors via RaP service
Journal
Microgel fabrication and tethering of SA-PD-L1
Authors: María M. Coronel, Haval Shirwan and Andrés J. García
Date: Jan 19, 2021，Views：1091
Original protocol used in research article：
Immunotherapy via PD-L1–presenting biomaterials leads to long-term islet graft survival
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.aba5573
Nano-LC-MS/MS analysis
Author: Wenqing Shui
Date: Jan 19, 2021，Views：1046
Original protocol used in research article：
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae
eLife, Mar 13, 2017; DOI: 10.7554/eLife.23816
Yeast microsome preparation
Author: Wenqing Shui
Date: Jan 19, 2021，Views：2159
Original protocol used in research article：
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae
eLife, Mar 13, 2017; DOI: 10.7554/eLife.23816
Collection of in vivo capacitated sperm
Authors: Lukas Ded and Jean-Ju Chung
Date: Jan 19, 2021，Views：1376
Original protocol used in research article：
3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice
eLife, Oct 20, 2020; DOI: 10.7554/eLife.62043
Flow cytometry analysis
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：984
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
Immunohistochemistry
Author: Hong-Ru Chen
Date: Jan 19, 2021，Views：972
Original protocol used in research article：
Fate mapping via CCR2-CreER mice reveals monocyte-to-microglia transition in development and neonatal stroke
Science Advances, Aug 25, 2020; DOI: 10.1126/sciadv.abb2119
Traumatic brain injury
Authors: Rebekah Mannix, Wen Li and Jianhua Qiu
Date: Jan 19, 2021，Views：1155
Original protocol used in research article：
BBB pathophysiology–independent delivery of siRNA in traumatic brain injury
Science Advances, Dec 31, 2020; DOI: 10.1126/sciadv.abd6889
CRISPR/Cas9-mediated targeting of TINF2 in RPE-1 cells
Author: Isabelle Schmutz
Date: Jan 19, 2021，Views：1448
Original protocol used in research article：
TINF2 is a haploinsufficient tumor suppressor that limits telomere length
eLife, Dec 1, 2020; DOI: 10.7554/eLife.61235
Calculation of the divergence rate
Author: Germán Plata
Date: Jan 18, 2021，Views：1135
Original protocol used in research article：
Molecular function limits divergent protein evolution on planetary timescales
eLife, Sep 18, 2019; DOI: 10.7554/eLife.39705
Cell lines and in vitro culture conditions
Authors: Qianru Jin, David H. Gracias and Lewis H. Romer
Date: Jan 17, 2021，Views：948
Original protocol used in research article：
Biomimetic human small muscular pulmonary arteries
Science Advances, Mar 24, 2020; DOI: 10.1126/sciadv.aaz2598
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