Protocols shared by authors via RaP service
Journal
STED imaging
Author: Monica M Sousa
Date: Dec 10, 2020，Views：909
Original protocol used in research article：
The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction
eLife, Mar 20, 2020; DOI: 10.7554/eLife.55471
Composite agarose-polyacrylamide gel electrophoresis (Composite AgPAGE)
Authors: Kirk Bergstrom, Xindi Shan and Lijun Xia
Date: Dec 9, 2020，Views：1070
Original protocol used in research article：
Proximal colon–derived O-glycosylated mucus encapsulates and modulates the microbiota
Science, Oct 23, 2020; DOI: 10.1126/science.aay7367
Adoptive cell transfer (ACT) and RNA-LPX treatment
Author: Katharina Reinhard
Date: Dec 9, 2020，Views：1058
Original protocol used in research article：
An RNA vaccine drives expansion and efficacy of claudin-CAR-T cells against solid tumors
Science, Jan 1, 2020; DOI: 10.1126/science.aay5967
Ex vivo live imaging
Authors: Anamika Sharma and Gaiti Hasan
Date: Dec 9, 2020，Views：1193
Original protocol used in research article：
Modulation of flight and feeding behaviours requires presynaptic IP3Rs in dopaminergic neurons
eLife, Nov 6, 2020; DOI: 10.7554/eLife.62297
Bacterial flow cytometry (BUGFlow)
Author: Anne-Katrin Pröbstel
Date: Dec 9, 2020，Views：1173
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
16S rRNA gene analysis
Author: Xiaoyuan Zhou
Date: Dec 9, 2020，Views：928
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
LNP preparation
Authors: Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2457
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
LNP transfection
Authors: Dan Peer, Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2779
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
Phenotyping of adoptively transferred and endogenous tumor-infiltrating CD8+ T cells in the B16F10 orthotopic melanoma model
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Dec 8, 2020，Views：914
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Flow cytometry dissociation protocol for Individual Drosophila brains
Author: Shyama Nandakumar
Date: Dec 7, 2020，Views：1103
Original protocol used in research article：
Polyploidy in the adult Drosophila brain
eLife, Aug 25, 2020; DOI: 10.7554/eLife.54385
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