Cell lines and in vitro culture conditions

Dear Dr. Zenhausern, 

    Thanks for your interest in our work and the questions. 

    The principle that how our 3D microvessels are formed from 2D film is based on the stress mismatch between bilayers of silicon monoxide and silicon dioxide. One layer has a tensile stress built in during physical vapor dposition, while the other layer is neutral in stress. This tensile stress in one layer and the neutral stress in another layer causes the rolling once the bilayer is released from the substrate. The 2nd part in the Materials and Methods section provides details in this fabrication process. 

    If you are interested in extending the construct into cellulose based materials, my suggestion is to see if you could prepare two types of cellulose based materials with different swelling ratio, and stack one layer on top of the other on a dissovable sacrifial layer on a substrate, and release this bilayer from the substrate by dissolving the sacrifical layer to see if they fold into a tubular or curved construct. 

    You could also refer to this paper from our lab before, where we used PEGDA based hydrogel with two different swelling ratios to build tubular constructs. https://doi.org/10.1089/ten.tec.2015.0442

    I would be happy to answer other questions you might have. Thank you!

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