Protocols shared by authors via RaP service
Journal
GeneWeld: a method for efficient targeted integration directed by short homology
Authors: Maura McGrail and Jeffrey Essner
Date: Nov 7, 2020，Views：1517
Original protocol used in research article：
Efficient targeted integration directed by short homology in zebrafish and mammalian cells
eLife, May 15, 2020; DOI: 10.7554/eLife.53968
Splicing efficiency and poly(A) tail length/site
Authors: Daniel R Larson and Murali Palangat
Date: Nov 7, 2020，Views：914
Original protocol used in research article：
Kinetic competition during the transcription cycle results in stochastic RNA processing
eLife, Oct 1, 2014; DOI: 10.7554/eLife.03939
T1RESS sequence design and scan parameters
Author: Robert R Edelman
Date: Nov 6, 2020，Views：836
Original protocol used in research article：
Twofold improved tumor-to-brain contrast using a novel T1 relaxation-enhanced steady-state (T1RESS) MRI technique
Science Advances, Oct 28, 2020; DOI: 10.1126/sciadv.abd1635
Nanogel preparation and characterization
Author: Xiao Xu
Date: Nov 6, 2020，Views：916
Original protocol used in research article：
Self-regulated hirudin delivery for anticoagulant therapy
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc0382
Crispr-CAS9 knockout of Piezo1
Author: Josefina Ines del Marmol
Date: Nov 5, 2020，Views：941
Original protocol used in research article：
Piezo1 forms a slowly-inactivating mechanosensory channel in mouse embryonic stem cells
eLife, Aug 22, 2018; DOI: 10.7554/eLife.33149
Assay protocols
Authors: Ali H. Alghamdi and Harry P. De Koning
Date: Nov 5, 2020，Views：1091
Original protocol used in research article：
Positively selected modifications in the pore of TbAQP2 allow pentamidine to enter Trypanosoma brucei
eLife, Aug 11, 2020; DOI: 10.7554/eLife.56416
Construction of the BiG Mito-Split-GFP strain
Authors: Roza Kucharczyk and Hubert Dominique Becker
Date: Nov 5, 2020，Views：1097
Original protocol used in research article：
Assigning mitochondrial localization of dual localized proteins using a yeast Bi-Genomic Mitochondrial-Split-GFP
eLife, Jul 13, 2020; DOI: 10.7554/eLife.56649
Single cell library preparation
Author: Christopher A Jackson
Date: Nov 5, 2020，Views：997
Original protocol used in research article：
Gene regulatory network reconstruction using single-cell RNA sequencing of barcoded genotypes in diverse environments
eLife, Jan 27, 2020; DOI: 10.7554/eLife.51254
Molecular Dynamics simulations
Authors: Alzbeta Tuerkova, Robert Vácha and Ivo Kabelka
Date: Nov 4, 2020，Views：2458
Original protocol used in research article：
Effect of helical kink in antimicrobial peptides on membrane pore formation
eLife, Mar 13, 2020; DOI: 10.7554/eLife.47946
Single-cell RNA sequencing of endosteal bone marrow cells
Author: Leilei Zhong
Date: Nov 3, 2020，Views：1113
Original protocol used in research article：
Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment
eLife, Apr 14, 2020; DOI: 10.7554/eLife.54695
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