Protocols shared by authors via RaP service
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Specificity scores
Authors: Silvia Domcke and Andrew J. Hill
Date: Nov 20, 2020，Views：964
Original protocol used in research article：
A human cell atlas of fetal chromatin accessibility
Science, Nov 13, 2020; DOI: 10.1126/science.aba7612
SARS-CoV-2 neutralization assay
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：1031
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
LION formulation of the repRNA vaccine
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：953
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
Computational prediction of RNA structures
Author: Yan Zhang
Date: Nov 19, 2020，Views：1003
Original protocol used in research article：
Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037
Promoter Sequences
Author: Tarik F. Haydar
Date: Nov 19, 2020，Views：970
Original protocol used in research article：
Transcriptional priming as a conserved mechanism of lineage diversification in the developing mouse and human neocortex
Science Advances, Nov 5, 2020; DOI: 10.1126/sciadv.abd2068
Single-molecule analysis of replication
Author: W. Liu
Date: Nov 19, 2020，Views：1053
Original protocol used in research article：
Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors
Science Advances, Nov 12, 2020; DOI: 10.1126/sciadv.abc3598
Phase separation using an optoDroplet system
Author: Yujie Sun
Date: Nov 18, 2020，Views：1043
Original protocol used in research article：
Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering
Science Advances, Apr 14, 2020; DOI: 10.1126/sciadv.aay6515
Determination of SS31 concentration in main organs of AKI mice
Author: Yongzhong Du
Date: Nov 17, 2020，Views：929
Original protocol used in research article：
ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abb7422
Integrin and transferrin recycling assay
Authors: Fangfei Qu and Vann Bennett
Date: Nov 17, 2020，Views：1862
Original protocol used in research article：
Ankyrin-B is a PI3P effector that promotes polarized α5β1-integrin recycling via recruiting RabGAP1L to early endosomes
eLife, Oct 8, 2016; DOI: 10.7554/eLife.20417
Skin penetration studies
Author: Abhirup Mandal
Date: Nov 17, 2020，Views：987
Original protocol used in research article：
Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.abb6049
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