Protocols shared by authors via RaP service
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Promoter Sequences
Author: Tarik F. Haydar
Date: Nov 19, 2020，Views：920
Original protocol used in research article：
Transcriptional priming as a conserved mechanism of lineage diversification in the developing mouse and human neocortex
Science Advances, Nov 5, 2020; DOI: 10.1126/sciadv.abd2068
Single-molecule analysis of replication
Author: W. Liu
Date: Nov 19, 2020，Views：996
Original protocol used in research article：
Two replication fork remodeling pathways generate nuclease substrates for distinct fork protection factors
Science Advances, Nov 12, 2020; DOI: 10.1126/sciadv.abc3598
Phase separation using an optoDroplet system
Author: Yujie Sun
Date: Nov 18, 2020，Views：999
Original protocol used in research article：
Nuclear actin regulates inducible transcription by enhancing RNA polymerase II clustering
Science Advances, Apr 14, 2020; DOI: 10.1126/sciadv.aay6515
Determination of SS31 concentration in main organs of AKI mice
Author: Yongzhong Du
Date: Nov 17, 2020，Views：884
Original protocol used in research article：
ROS-responsive chitosan-SS31 prodrug for AKI therapy via rapid distribution in the kidney and long-term retention in the renal tubule
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abb7422
Integrin and transferrin recycling assay
Authors: Fangfei Qu and Vann Bennett
Date: Nov 17, 2020，Views：1742
Original protocol used in research article：
Ankyrin-B is a PI3P effector that promotes polarized α5β1-integrin recycling via recruiting RabGAP1L to early endosomes
eLife, Oct 8, 2016; DOI: 10.7554/eLife.20417
Skin penetration studies
Author: Abhirup Mandal
Date: Nov 17, 2020，Views：960
Original protocol used in research article：
Treatment of psoriasis with NFKBIZ siRNA using topical ionic liquid formulations
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.abb6049
Physiological age
Authors: Xia Li and Sara Hägg
Date: Nov 17, 2020，Views：1654
Original protocol used in research article：
Longitudinal trajectories, correlations and mortality associations of nine biological ages across 20-years follow-up
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51507
eIF2α phosphorylation
Authors: Yize Li, Robert H Silverman and Susan R Weiss
Date: Nov 17, 2020，Views：921
Original protocol used in research article：
Ribonuclease L mediates the cell-lethal phenotype of double-stranded RNA editing enzyme ADAR1 deficiency in a human cell line
eLife, Mar 31, 2017; DOI: 10.7554/eLife.25687
Generation of VEGF gradients using vascular bioink
Author: Fiona Freeman
Date: Nov 17, 2020，Views：913
Original protocol used in research article：
3D bioprinting spatiotemporally defined patterns of growth factors to tightly control tissue regeneration
Science Advances, Aug 14, 2020; DOI: 10.1126/sciadv.abb5093
Lactic acid treatment in T cell culture
Author: Franziska M. Uhl
Date: Nov 16, 2020，Views：870
Original protocol used in research article：
Metabolic reprogramming of donor T cells enhances graft-versus-leukemia effects in mice and humans
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.abb8969
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