Protocols shared by authors via RaP service
Journal
Y-maze, modified and spontaneous alternation task version
Authors: Da Hee Jung and Byung Tae Choi
Date: Nov 23, 2020，Views：5043
Original protocol used in research article：
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359
WGCNA network analysis
Author: Kathleen Greenham
Date: Nov 23, 2020，Views：1129
Original protocol used in research article：
Temporal network analysis identifies early physiological and transcriptomic indicators of mild drought in Brassica rapa
eLife, Aug 18, 2017; DOI: 10.7554/eLife.29655
‘On-bead’ peptidisc reconstitution
Author: Franck Duong
Date: Nov 23, 2020，Views：1000
Original protocol used in research article：
The Peptidisc, a simple method for stabilizing membrane proteins in detergent-free solution
eLife, Aug 15, 2018; DOI: 10.7554/eLife.34085
Western blot analysis of cultured fibroblasts
Author: Philip S. Low
Date: Nov 22, 2020，Views：797
Original protocol used in research article：
Targeted inhibition of PI3 kinase/mTOR specifically in fibrotic lung fibroblasts suppresses pulmonary fibrosis in experimental models
Science Translational Medicine, Oct 27, 2020; DOI: 10.1126/scitranslmed.aay3724
Recombinant IRF5 dimerization assay - protein preparation
Author: Gang Chen
Date: Nov 20, 2020，Views：1108
Original protocol used in research article：
Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization
Science Advances, May 14, 2020; DOI: 10.1126/sciadv.aay1057
Calculating scores and classifications
Author: Melissa Chiasson
Date: Nov 20, 2020，Views：826
Original protocol used in research article：
Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact
eLife, Sep 1, 2020; DOI: 10.7554/eLife.58026
Specificity scores
Authors: Silvia Domcke and Andrew J. Hill
Date: Nov 20, 2020，Views：921
Original protocol used in research article：
A human cell atlas of fetal chromatin accessibility
Science, Nov 13, 2020; DOI: 10.1126/science.aba7612
SARS-CoV-2 neutralization assay
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：933
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
LION formulation of the repRNA vaccine
Author: Jesse H. Erasmus
Date: Nov 20, 2020，Views：918
Original protocol used in research article：
An Alphavirus-derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates
Science Translational Medicine, Aug 4, 2020; DOI: 10.1126/scitranslmed.abc9396
Computational prediction of RNA structures
Author: Yan Zhang
Date: Nov 19, 2020，Views：938
Original protocol used in research article：
Operon mRNAs are organized into ORF-centric structures that predict translation efficiency
eLife, Jan 31, 2017; DOI: 10.7554/eLife.22037
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