Protocols shared by authors via RaP service
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Evaluating T cell escape in epitopes previously defined as reactive in clade B consensus mapping - Protocol for Elispot, Peptide handling
Authors: Joanna A Warren and Nilu Goonetilleke
Date: Dec 7, 2020，Views：1052
Original protocol used in research article：
The HIV-1 latent reservoir is largely sensitive to circulating T cells
eLife, Oct 6, 2020; DOI: 10.7554/eLife.57246
Transfection, pool maintenance, and drug addition
Authors: Raghuvir Viswanatha and Norbert Perrimon
Date: Dec 7, 2020，Views：1029
Original protocol used in research article：
Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36333
Stimuli
Author: Paolo Ossola
Date: Dec 7, 2020，Views：947
Original protocol used in research article：
Belief updating in bipolar disorder predicts time of recurrence
eLife, Nov 10, 2020; DOI: 10.7554/eLife.58891
Single-cell RNA-seq analysis of public 10x data
Authors: Jean-Baptiste Gorin, Vincent Wu, Son Nguyen and Marcus Buggert
Date: Dec 7, 2020，Views：860
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Isolation of single cells from cryoinjured hearts
Author: Jeroen Bakkers
Date: Dec 5, 2020，Views：946
Original protocol used in research article：
Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
eLife, Dec 23, 2019; DOI: 10.7554/eLife.50163
Demographic history with dadi
Author: Ira Cooke
Date: Dec 4, 2020，Views：899
Original protocol used in research article：
Genomic signatures in the coral holobiont reveal host adaptations driven by Holocene climate change and reef specific symbionts
Science Advances, Nov 26, 2020; DOI: 10.1126/sciadv.abc6318
Human specimens, neuropathological evaluation, and criteria of diagnosis
Author: Eliezer Masliah
Date: Dec 4, 2020，Views：893
Original protocol used in research article：
LRRK2 mediates microglial neurotoxicity via NFATc2 in rodent models of synucleinopathies
Science Translational Medicine, Oct 13, 2020; DOI: 10.1126/scitranslmed.aay0399
ChIP-seq
Author: Randall H Morse
Date: Dec 4, 2020，Views：961
Original protocol used in research article：
Role of the pre-initiation complex in Mediator recruitment and dynamics
eLife, Dec 12, 2018; DOI: 10.7554/eLife.39633
Primers for Quantitative real-time PCR
Authors: Shunxing Rong and Jay D Horton
Date: Dec 4, 2020，Views：1175
Original protocol used in research article：
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
eLife, Feb 28, 2017; DOI: 10.7554/eLife.25015
Programming and reading schemes of the memristor-based system
Author: Jianshi Tang
Date: Dec 4, 2020，Views：909
Original protocol used in research article：
Multichannel parallel processing of neural signals in memristor arrays
Science Advances, Oct 8, 2020; DOI: 10.1126/sciadv.abc4797
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