Protocols shared by authors via RaP service
Journal
APAP-ALF patient plasma analysis
Authors: Katelyn Arnold and Jian Liu
Date: Dec 2, 2020，Views：890
Original protocol used in research article：
Design of anti-inflammatory heparan sulfate to protect against acetaminophen-induced acute liver failure
Science Translational Medicine, Mar 17, 2020; DOI: 10.1126/scitranslmed.aav8075
Whole-Mount in situ hybridization
Author: Andrea Elizabeth Wills
Date: Dec 1, 2020，Views：906
Original protocol used in research article：
Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors
eLife, Apr 27, 2020; DOI: 10.7554/eLife.52648
Primers information for quantitative PCR
Authors: Xiao Ma and Xiaolei Xu
Date: Dec 1, 2020，Views：1040
Original protocol used in research article：
Retinoid X receptor alpha is a spatiotemporally predominant therapeutic target for anthracycline-induced cardiotoxicity
Science Advances, Jan 28, 2020; DOI: 10.1126/sciadv.aay2939
Fecal microbiota 16S rRNA gene sequencing
Author: Jakob Stokholm
Date: Dec 1, 2020，Views：849
Original protocol used in research article：
Delivery mode and gut microbial changes correlate with an increased risk of childhood asthma
Science Translational Medicine, Nov 10, 2020; DOI: 10.1126/scitranslmed.aax9929
Marble burying test
Author: Huda Y Zoghbi
Date: Nov 30, 2020，Views：1156
Original protocol used in research article：
Restoration of Mecp2 expression in GABAergic neurons is sufficient to rescue multiple disease features in a mouse model of Rett syndrome
eLife, Jun 21, 2016; DOI: 10.7554/eLife.14198
NMR spectroscopy and structure calculations
Author: Edward K. Gilding
Date: Nov 30, 2020，Views：843
Original protocol used in research article：
Neurotoxic peptides from the venom of the giant Australian stinging tree
Science Advances, Sep 16, 2020; DOI: 10.1126/sciadv.abb8828
Analysis of Ca2+ transients
Author: Katsutoshi Mizuno
Date: Nov 30, 2020，Views：899
Original protocol used in research article：
Role of Ca2+ transients at the node of the mouse embryo in breaking of left-right symmetry
Science Advances, Jul 21, 2020; DOI: 10.1126/sciadv.aba1195
Enzymatic dispersal of Cerebral artery SMCs
Author: Scott Earley
Date: Nov 30, 2020，Views：853
Original protocol used in research article：
TRPML1 channels initiate Ca2+ sparks in vascular smooth muscle cells
Science Signaling, Jun 22, 2020; DOI: 10.1126/scisignal.aba1015
Human NP cell isolation and culture
Author: Yongxin Ren
Date: Nov 30, 2020，Views：1053
Original protocol used in research article：
p16 deficiency attenuates intervertebral disc degeneration by adjusting oxidative stress and nucleus pulposus cell cycle
eLife, Mar 3, 2020; DOI: 10.7554/eLife.52570
Single cell ATAC-seq
Authors: Enric Llorens-Bobadilla and Jonas Frisén
Date: Nov 29, 2020，Views：953
Original protocol used in research article：
A latent lineage potential in resident neural stem cells enables spinal cord repair
Science, Oct 1, 2020; DOI: 10.1126/science.abb8795
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