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Yeast microsome preparation

WS Wenqing Shui

Last updated date: Jan 19, 2021 Views: 1864 Forks: 0

original An abbreviated version of this protocol was published in eLife in Mar, 2017
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae
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First author of this paper (Dr. Xingbo Wang) from Junjie Hu group performed the microsome preparation as described in the Methods and my group continued in protein extraction, digestion and nano-LC/MS analysis. Please contact Dr. Hu for a more detailed protocol if needed. Sorry I cannot be of more help for this request.


-Sincerely yours,

Wenqing Shui  

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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Shui, W(2021). Yeast microsome preparation. Bio-protocol Preprint. bio-protocol.org/prep764.
  2. Wang, X., Li, S., Wang, H., Shui, W. and Hu, J.(2017). Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae. eLife. DOI: 10.7554/eLife.23816

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