This protocol was submitted by the author(s) of the original paper via Bio-protocol's "Request a Protocol" feature.
Original Research Paper Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
DOI: 10.1126/sciadv.abd5575
Science Advances , Nov 25, 2020
Related protocols in the same article
PBMC processing and stimulations
Thanaphong Phongpreecha    Rosemary Fernandez    Thomas Montine    
Original Research Paper
Last updated date: Dec 24, 2020 View: 342


Recipes

1.Complete RPMI (Hyclone RPMI-1640 Medium)

RPMI with 10% FBS

Pencillin/streptomycin

Glutamine

2.CyFACS buffer

1x CyPBS PBS with 0.1% BSA, and 0.05% Na azide

Made in MilliQ water

Note: Do not use FBS!


References
  1. Bendall SC, Simonds EF, Qiu P, Amirel AD, Krutzik PO, Finck R, Bruggner RV, Melamed R, Trejo A, Ornatsky OI, Balderas RS, Plevritis SK, Sachs K, Pe’er D, Tanner SD, Nolan GP. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011; 332(6030):687–696. [PubMed: 21551058]

  2. Rosemary Fernandez and Holden Maecker. Cytokine-Stimulated Phosphoflow of PBMC using CyTOF mass cytometry. Bio Protoc 2015 Jun; 5(11)

How to cite: Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
1. Phongpreecha, T. , Fernandez, R. and Montine, T. (2020). PBMC processing and stimulations. Bio-protocol. bio-protocol.org/prep721.
2. Phongpreecha, T., Fernandez, R., Mrdjen, D., Culos, A., Gajera, C., Wawro, A., Stanley, N., Gaudilliere, B., Poston, K., Aghaeepour, N. and Montine, T.(2020). Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases. Science Advances 6(48). DOI: 10.1126/sciadv.abd5575
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