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Preprint

Primers for Quantitative real-time PCR

SR Shunxing Rong
JH Jay D Horton

Last updated date: Dec 4, 2020 Views: 1137 Forks: 0

original An abbreviated version of this protocol was published in eLife in Feb, 2017
Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice
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Primers
GenesForwardReverse
SREBP-2GCGTTCTGGAGACCATGGA ACAAAGTTGCTCTGAAAACAAATCA
SREBP-1aGGCCGAGATGTGCGAACT TTGTTGATGAGCTGGAGCATGT
SREBP-1cGGAGCCATGGATTGCACATT GGCCCGGGAAGTCACTGT
LDLRAGGCTGTGGGCTCCATAGG TGCGGTCCAGGGTCATCT
HMG-CoA   synthaseGCCGTGAACTGGGTCGAA GCATATATAGCAATGTCTCCTGCAA
HMG-CoA   reductaseCTTGTGGAATGCCTTGTGATTG AGCCGAAGCAGCACATGAT
Squalene   synthaseCCAACTCAATGGGTCTGTTCCT TGGCTTAGCAAAGTCTTCCAACT
Acetyl-CoA   Carboxylase1TGGACAGACTGATCGCAGAGAAAG TGGAGAGCCCCACACACA
Fatty acid   synthaseGCTGCGGAAACTTCAGGAAAT AGAGACGTGTCACTCCTGGACTT
ELOVL6TGTACGCTGCCTTTATCTTTGG GCGGCTTCCGAAGTTCAA
Stearoyl-CoA   desaturase 1CCGGAGACCCCTTAGATCGA TAGCCTGTAAAAGATTTCTGCAAACC
PNPLA3TGGCTACAATGGGCGACAT GGCCCAAGTATTTACCTGGTAGAG
CHREBPCCTTCGCCAACTCAGCACTT TGGCTTGCTCAGGCACAA
GlucokinaseCCGTGATCCGGGAAGAGAA GGGAAACCTGACAGGGATGAG
G6PDGAACGCAAAGCTGAAGTGAGACT TCATTACGCTTGCACTGTTGGT
PEPCKCCACAGCTGCTGCAGAACA GAAGGGTCGCATGGCAAA
ApoBCGTGGGCTCCAGCATTCTA TCACCAGTCATTTCTGCCTTTG
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How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Rong, S and Horton, J(2020). Primers for Quantitative real-time PCR. Bio-protocol Preprint. bio-protocol.org/prep678.
  2. Rong, S., Cortés, V. A., Rashid, S., Anderson, N. N., McDonald, J. G., Liang, G., Moon, Y., Hammer, R. E. and Horton, J. D.(2017). Expression of SREBP-1c Requires SREBP-2-mediated Generation of a Sterol Ligand for LXR in Livers of Mice. eLife. DOI: 10.7554/eLife.25015

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