Protocols shared by authors via RaP service
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Imaging Cytosolic Calcium in T Lymphocytes: a protocol for use with genetically encoded or chemical Ca2+ indicators
Author: Michael D Cahalan
Date: Mar 10, 2021，Views：1158
Original protocol used in research article：
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
eLife, Dec 14, 2017; DOI: 10.7554/eLife.32417
Petri dish hygrotaxis arena
Author: Jennifer S Sun
Date: Mar 9, 2021，Views：871
Original protocol used in research article：
Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249
Redox potential measurements
Author: Aspen T Reese
Date: Mar 8, 2021，Views：934
Original protocol used in research article：
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987
Recovery cohousing following antibiotic treatment
Author: Aspen T Reese
Date: Mar 8, 2021，Views：856
Original protocol used in research article：
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987
Construction of plasmids
Author: Eric Klavins
Date: Mar 8, 2021，Views：955
Original protocol used in research article：
Synthetic hormone-responsive transcription factors can monitor and re-program plant development
eLife, May 1, 2018; DOI: 10.7554/eLife.34702
Protein expression and purification of the cysteine-rich domain of Frizzled receptors
Author: Tao-Hsin Chang
Date: Mar 7, 2021，Views：1065
Original protocol used in research article：
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan
eLife, Jul 9, 2015; DOI: 10.7554/eLife.06554
Kmer mapping
Author: Bernadette C Young
Date: Mar 7, 2021，Views：1718
Original protocol used in research article：
Panton–Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS
eLife, Feb 22, 2019; DOI: 10.7554/eLife.42486
Measurement of the labile iron pool
Author: Jumpei Ito
Date: Mar 5, 2021，Views：1629
Original protocol used in research article：
Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice
eLife, Feb 2, 2021; DOI: 10.7554/eLife.62174
crRNA selection
Authors: Francois Kroll and Jason Rihel
Date: Mar 5, 2021，Views：1269
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
Information flow analysis
Author: Lucie Delemotte
Date: Mar 4, 2021，Views：782
Original protocol used in research article：
Calmodulin acts as a state-dependent switch to control a cardiac potassium channel opening
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abd6798
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