Protocols shared by authors via RaP service
Journal
Calculation of dice scores
Authors: Yunyun Han and Peng Fei
Date: Apr 1, 2021，Views：867
Original protocol used in research article：
Bi-channel image registration and deep-learning segmentation (BIRDS) for efficient, versatile 3D mapping of mouse brain
eLife, Jan 18, 2021; DOI: 10.7554/eLife.63455
Differentiation of human and mouse THGM cells in vitro
Authors: Javad Rasouli, Bogoljub Ciric and Abdolmohamad Rostami
Date: Apr 1, 2021，Views：956
Original protocol used in research article：
A distinct GM-CSF+ T helper cell subset requires T-bet to adopt a TH1 phenotype and promote neuroinflammation
Science Immunology, Oct 22, 2020; DOI: 10.1126/sciimmunol.aba9953
PDC isolation and culture
Authors: Li-Wei Dong, Ye-Xiong Tan and Hong-Yang Wang
Date: Apr 1, 2021，Views：851
Original protocol used in research article：
PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma
Science Translational Medicine, Sep 22, 2020; DOI: 10.1126/scitranslmed.aay0152
Activity recording
Authors: Yusaku Hontani, Fei Xia and Chris Xu
Date: Mar 31, 2021，Views：988
Original protocol used in research article：
Multicolor three-photon fluorescence imaging with single-wavelength excitation deep in mouse brain
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abf3531
Optogenetic control of CA production
Authors: Meng C Wang and Jeffrey J Tabor
Date: Mar 31, 2021，Views：878
Original protocol used in research article：
Optogenetic control of gut bacterial metabolism to promote longevity
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56849
Preparation of αSyn PFFs
Author: Jin-Song Bian
Date: Mar 30, 2021，Views：919
Original protocol used in research article：
Anti–Na+/K+-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na+/K+-ATPase α1–dependent autophagy
Science Advances, Jan 26, 2021; DOI: 10.1126/sciadv.abc5062
ND-labeling C. elegans worms
Authors: Masazumi Fujiwara, Eriko Kage-Nakadai and Simo Sun
Date: Mar 30, 2021，Views：848
Original protocol used in research article：
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636
Zebrafish retinal dissociation, cell sorting, and methanol fixation
Authors: Patrick Boyd and David R. Hyde
Date: Mar 29, 2021，Views：954
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Cortical excitatory neuron differentiation
Author: Dina Simkin
Date: Mar 29, 2021，Views：1162
Original protocol used in research article：
Dyshomeostatic modulation of Ca2+-activated K+ channels in a human neuronal model of KCNQ2 encephalopathy
eLife, Feb 5, 2021; DOI: 10.7554/eLife.64434
Parasite strains, culturing and resistance selection
Author: Eva S Istvan
Date: Mar 28, 2021，Views：934
Original protocol used in research article：
Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529
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