Protocols shared by authors via RaP service
Journal
Generation of transgenic lines
Authors: Oriol Llorà-Batlle and Alfred Cortés
Date: Mar 17, 2021，Views：786
Original protocol used in research article：
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz5057
Isolation of T-cell subpopulations
Authors: Anastasia A Minervina and Mikhail V Pogorelyy
Date: Mar 17, 2021，Views：1368
Original protocol used in research article：
Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
eLife, Feb 21, 2020; DOI: 10.7554/eLife.53704
Strand-specific lncRNA sequencing and analysis
Authors: Shiqi Luo and Xin Zhou
Date: Mar 17, 2021，Views：895
Original protocol used in research article：
Gene reuse facilitates rapid radiation and independent adaptation to diverse habitats in the Asian honeybee
Science Advances, Dec 17, 2020; DOI: 10.1126/sciadv.abd3590
Generate dot surface of the channel hole for CALHM1 using HOLE program
Authors: Xue Yang and Yuequan Shen
Date: Mar 16, 2021，Views：928
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
MS/MS biclustering and molecular networking
Author: Dapeng Li
Date: Mar 16, 2021，Views：768
Original protocol used in research article：
Information theory tests critical predictions of plant defense theory for specialized metabolism
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz0381
Protein expression and purification
Author: Yue Ren
Date: Mar 16, 2021，Views：949
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
scVelo
Author: Bryan Marsh
Date: Mar 15, 2021，Views：2232
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
Oil-Red-O staining to determine the amount of stored triglyceride in Caenorhabditis elegans
Authors: Avijit Mallick and Bhagwati P Gupta
Date: Mar 15, 2021，Views：864
Original protocol used in research article：
Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans
microPublication Biology, Jul 21, 2020; DOI: 10.17912/micropub.biology.000281
Calculation of VISTA module score
Author: Yanding Zhao
Date: Mar 15, 2021，Views：842
Original protocol used in research article：
VISTA is a checkpoint regulator for naïve T cell quiescence and peripheral tolerance
Science, Jan 16, 2020; DOI: 10.1126/science.aay0524
Virus neutralization assay
Authors: Yasumasa Iwatani and Hiroshi Murakami
Date: Mar 15, 2021，Views：969
Original protocol used in research article：
Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916
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