Protocols shared by authors via RaP service
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Protein expression and purification
Authors: Claire Gorby and Ignacio Moraga
Date: Mar 11, 2021，Views：884
Original protocol used in research article：
Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses
Science Signaling, Sep 14, 2020; DOI: 10.1126/scisignal.abc0653
Automated filopodia analysis software
Author: Johannes Stegmaier
Date: Mar 10, 2021，Views：918
Original protocol used in research article：
Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
eLife, Jul 31, 2018; DOI: 10.7554/eLife.36953
Primary mouse EC isolation
Authors: Nora Yucel and Zoltan Arany
Date: Mar 10, 2021，Views：936
Original protocol used in research article：
Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
eLife, Dec 14, 2020; DOI: 10.7554/eLife.55730
Imaging Cytosolic Calcium in T Lymphocytes: a protocol for use with genetically encoded or chemical Ca2+ indicators
Author: Michael D Cahalan
Date: Mar 10, 2021，Views：1111
Original protocol used in research article：
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
eLife, Dec 14, 2017; DOI: 10.7554/eLife.32417
Petri dish hygrotaxis arena
Author: Jennifer S Sun
Date: Mar 9, 2021，Views：844
Original protocol used in research article：
Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249
Redox potential measurements
Author: Aspen T Reese
Date: Mar 8, 2021，Views：910
Original protocol used in research article：
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987
Recovery cohousing following antibiotic treatment
Author: Aspen T Reese
Date: Mar 8, 2021，Views：830
Original protocol used in research article：
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987
Construction of plasmids
Author: Eric Klavins
Date: Mar 8, 2021，Views：934
Original protocol used in research article：
Synthetic hormone-responsive transcription factors can monitor and re-program plant development
eLife, May 1, 2018; DOI: 10.7554/eLife.34702
Protein expression and purification of the cysteine-rich domain of Frizzled receptors
Author: Tao-Hsin Chang
Date: Mar 7, 2021，Views：1031
Original protocol used in research article：
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan
eLife, Jul 9, 2015; DOI: 10.7554/eLife.06554
Kmer mapping
Author: Bernadette C Young
Date: Mar 7, 2021，Views：1676
Original protocol used in research article：
Panton–Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS
eLife, Feb 22, 2019; DOI: 10.7554/eLife.42486
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