Protocols shared by authors via RaP service
Journal
Computational methods
Authors: Lizhi Liu and Sheng Chen
Date: Mar 15, 2021，Views：835
Original protocol used in research article：
Mechanical penetration of β-lactam–resistant Gram-negative bacteria by programmable nanowires
Science Advances, Jul 2, 2020; DOI: 10.1126/sciadv.abb9593
Headloop PCR
Author: Gareth Thomas Powell
Date: Mar 15, 2021，Views：2410
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
ChIP-seq
Author: Wen Tang
Date: Mar 15, 2021，Views：956
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
De novo protein design
Author: Anastassia A. Vorobieva
Date: Mar 15, 2021，Views：914
Original protocol used in research article：
De novo design of transmembrane β barrels
Science, Feb 18, 2021; DOI: 10.1126/science.abc8182
Organoid experiments culture & seeding
Authors: Wouter R. Karthaus, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：1017
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Prostate isolation and enzymatic digestion
Authors: Wouter R. Karthaus, Matan Hofree, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：994
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Single-cell dissociation and RNA sequencing
Author: Michael L Wallace
Date: Mar 11, 2021，Views：896
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
Protein expression and purification
Authors: Claire Gorby and Ignacio Moraga
Date: Mar 11, 2021，Views：908
Original protocol used in research article：
Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses
Science Signaling, Sep 14, 2020; DOI: 10.1126/scisignal.abc0653
Automated filopodia analysis software
Author: Johannes Stegmaier
Date: Mar 10, 2021，Views：941
Original protocol used in research article：
Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
eLife, Jul 31, 2018; DOI: 10.7554/eLife.36953
Primary mouse EC isolation
Authors: Nora Yucel and Zoltan Arany
Date: Mar 10, 2021，Views：960
Original protocol used in research article：
Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
eLife, Dec 14, 2020; DOI: 10.7554/eLife.55730
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