Protocols shared by authors via RaP service
Journal
Protein extraction from sucrose gradient fractions
Author: Massimiliano Clamer
Date: Mar 19, 2021，Views：974
Original protocol used in research article：
One-shot analysis of translated mammalian lncRNAs with AHARIBO
eLife, Feb 17, 2021; DOI: 10.7554/eLife.59303
Bacterial isolation, genome amplification and DNA extraction
Authors: Panagiotis Sapountzis and Panagiotis Sapountzis
Date: Mar 19, 2021，Views：954
Original protocol used in research article：
Reconstructing the functions of endosymbiotic Mollicutes in fungus-growing ants
eLife, Nov 20, 2018; DOI: 10.7554/eLife.39209
Cryo-EM data collection
Authors: Sunbin Deng and Ronen Marmorstein
Date: Mar 18, 2021，Views：856
Original protocol used in research article：
Molecular basis for N-terminal alpha-synuclein acetylation by human NatB
eLife, Sep 4, 2020; DOI: 10.7554/eLife.57491
PIV
Authors: James F Pelletier and Timothy J Mitchison
Date: Mar 18, 2021，Views：1041
Original protocol used in research article：
Co-movement of astral microtubules, organelles and F-actin by dynein and actomyosin forces in frog egg cytoplasm
eLife, Dec 7, 2020; DOI: 10.7554/eLife.60047
Characterization techniques and analyses
Author: Hadi Shafiee
Date: Mar 18, 2021，Views：870
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
Reduced silver staining
Authors: Gabriella Hannah Wolff and Nicholas James Strausfeld
Date: Mar 18, 2021，Views：886
Original protocol used in research article：
An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889
Cell Clustering and cluster identification
Author: Michael L Wallace
Date: Mar 18, 2021，Views：968
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
First and second strand cDNA synthesis and pre-amplification
Author: Arthur M Talman
Date: Mar 18, 2021，Views：968
Original protocol used in research article：
Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33105
Generation of transgenic lines
Authors: Oriol Llorà-Batlle and Alfred Cortés
Date: Mar 17, 2021，Views：801
Original protocol used in research article：
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz5057
Isolation of T-cell subpopulations
Authors: Anastasia A Minervina and Mikhail V Pogorelyy
Date: Mar 17, 2021，Views：1401
Original protocol used in research article：
Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
eLife, Feb 21, 2020; DOI: 10.7554/eLife.53704
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.