Protocols shared by authors via RaP service
Journal
RNA-seq
Authors: Hsin-I Jen and Andrew K Groves
Date: Apr 16, 2021，Views：910
Original protocol used in research article：
Transcriptomic and epigenetic regulation of hair cell regeneration in the mouse utricle and its potentiation by Atoh1
eLife, Apr 29, 2019; DOI: 10.7554/eLife.44328
Expression, purification and on-bead reconstitution of MsbA and MscS into peptidiscs
Authors: Gabriella Angiulli, Franck Duong Van Hoa and Thomas Walz
Date: Apr 16, 2021，Views：1031
Original protocol used in research article：
New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins
eLife, Mar 3, 2020; DOI: 10.7554/eLife.53530
Open-field test
Authors: Byung Tae Choi and Da Hee Jung
Date: Apr 16, 2021，Views：1781
Original protocol used in research article：
Therapeutic effects of anodal transcranial direct current stimulation in a rat model of ADHD
eLife, Sep 21, 2020; DOI: 10.7554/eLife.56359
Chronic mild stress
Authors: Huafu Chen and Zili You
Date: Apr 15, 2021，Views：967
Original protocol used in research article：
IL4-driven microglia modulate stress resilience through BDNF-dependent neurogenesis
Science Advances, Mar 17, 2021; DOI: 10.1126/sciadv.abb9888
Computational design of antibody nanocages (AbCs)
Authors: Robby Divine and David Baker
Date: Apr 15, 2021，Views：925
Original protocol used in research article：
Designed proteins assemble antibodies into modular nanocages
Science, Apr 2, 2021; DOI: 10.1126/science.abd9994
Synthesis of germanium nanospheres as trappable optical probes (GeNTOPs)
Authors: Swathi Sudhakar, Viktoria Wedler and Erik Schäffer
Date: Apr 15, 2021，Views：989
Original protocol used in research article：
Germanium nanospheres for ultraresolution picotensiometry of kinesin motors
Science, Feb 11, 2021; DOI: 10.1126/science.abd9944
B cell and T cell phenotyping
Author: Guy Gorochov
Date: Apr 14, 2021，Views：1040
Original protocol used in research article：
IgA dominates the early neutralizing antibody response to SARS-CoV-2
Science Translational Medicine, Jan 20, 2021; DOI: 10.1126/scitranslmed.abd2223
Hemolymph-brain barrier permeability
Author: Amita Sehgal
Date: Apr 13, 2021，Views：934
Original protocol used in research article：
Endocytosis at the Drosophila blood–brain barrier as a function for sleep
eLife, Nov 26, 2018; DOI: 10.7554/eLife.43326
RIME
Authors: Dylan A. Reid and Fred H. Gage
Date: Apr 12, 2021，Views：926
Original protocol used in research article：
Incorporation of a nucleoside analog maps genome repair sites in postmitotic human neurons
Science, Apr 2, 2021; DOI: 10.1126/science.abb9032
Cell treatments
Authors: Dylan A. Reid and Fred H. Gage
Date: Apr 12, 2021，Views：914
Original protocol used in research article：
Incorporation of a nucleoside analog maps genome repair sites in postmitotic human neurons
Science, Apr 2, 2021; DOI: 10.1126/science.abb9032
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