Protocols shared by authors via RaP service
Journal
Optogenetic control of CA production
Authors: Meng C Wang and Jeffrey J Tabor
Date: Mar 31, 2021，Views：843
Original protocol used in research article：
Optogenetic control of gut bacterial metabolism to promote longevity
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56849
Preparation of αSyn PFFs
Author: Jin-Song Bian
Date: Mar 30, 2021，Views：878
Original protocol used in research article：
Anti–Na+/K+-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na+/K+-ATPase α1–dependent autophagy
Science Advances, Jan 26, 2021; DOI: 10.1126/sciadv.abc5062
ND-labeling C. elegans worms
Authors: Masazumi Fujiwara, Eriko Kage-Nakadai and Simo Sun
Date: Mar 30, 2021，Views：783
Original protocol used in research article：
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636
Zebrafish retinal dissociation, cell sorting, and methanol fixation
Authors: Patrick Boyd and David R. Hyde
Date: Mar 29, 2021，Views：904
Original protocol used in research article：
Gene regulatory networks controlling vertebrate retinal regeneration
Science, Oct 1, 2020; DOI: 10.1126/science.abb8598
Cortical excitatory neuron differentiation
Author: Dina Simkin
Date: Mar 29, 2021，Views：1002
Original protocol used in research article：
Dyshomeostatic modulation of Ca2+-activated K+ channels in a human neuronal model of KCNQ2 encephalopathy
eLife, Feb 5, 2021; DOI: 10.7554/eLife.64434
Parasite strains, culturing and resistance selection
Author: Eva S Istvan
Date: Mar 28, 2021，Views：881
Original protocol used in research article：
Plasmodium Niemann-Pick type C1-related protein is a druggable target required for parasite membrane homeostasis
eLife, Mar 19, 2019; DOI: 10.7554/eLife.40529
PIV analysis
Author: HaiYang Wang
Date: Mar 28, 2021，Views：970
Original protocol used in research article：
Symmetry breaking in hydrodynamic forces drives meiotic spindle rotation in mammalian oocytes
Science Advances, Apr 3, 2020; DOI: 10.1126/sciadv.aaz5004
Spinning-disc confocal and 2P3A-DSLM imaging
Authors: Jia Zhao, Yanmei Liu and Liangyi Chen
Date: Mar 27, 2021，Views：1114
Original protocol used in research article：
In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540
Phylogenetic analysis of sleep-relevant genes
Authors: Junko Kusumi, Hiroyuki J. Kanaya and Taichi Q. Itoh
Date: Mar 27, 2021，Views：798
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Ce3D+ and Ce3D++ Tissue Clearing
Authors: Matthew J. Anderson, Valentin Magidson and Mark Lewandoski
Date: Mar 26, 2021，Views：1229
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
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