Protocols shared by authors via RaP service
Journal
Single-nucleus RNA-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 7, 2021，Views：1035
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
Microtubule co-sedimentation from worm extract
Author: Shaohe Wang
Date: Apr 7, 2021，Views：851
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Extending 3' UTRs in the transcriptome index
Authors: Lauren M Saunders, Cole Trapnell and David M Parichy
Date: Apr 7, 2021，Views：1001
Original protocol used in research article：
Thyroid hormone regulates distinct paths to maturation in pigment cell lineages
eLife, May 29, 2019; DOI: 10.7554/eLife.45181
Cell transformation assay
Authors: Karoliina Stefanius and Kim Orth
Date: Apr 7, 2021，Views：982
Original protocol used in research article：
Human pancreatic cancer cell exosomes, but not human normal cell exosomes, act as an initiator in cell transformation
eLife, May 28, 2019; DOI: 10.7554/eLife.40226
In situ hybridization
Authors: Kaiwen Kam and Carolina Thörn Perez
Date: Apr 7, 2021，Views：902
Original protocol used in research article：
Opioids modulate an emergent rhythmogenic process to depress breathing
eLife, Dec 16, 2019; DOI: 10.7554/eLife.50613
Luciferase assay
Authors: Lihui Han and dapeng ma
Date: Apr 7, 2021，Views：937
Original protocol used in research article：
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834
IP and immunoblot analysis
Authors: Lihui Han and Dapeng Ma
Date: Apr 7, 2021，Views：896
Original protocol used in research article：
Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response
Science Advances, Mar 24, 2021; DOI: 10.1126/sciadv.abc1834
RNA-seq and differential expression power analysis
Author: Benjamin Jung Fair
Date: Apr 6, 2021，Views：1053
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Confocal microscopy, live cell imaging, and intravital microscopy
Authors: Sang Hun Lee and David L. Sacks
Date: Apr 5, 2021，Views：994
Original protocol used in research article：
M2-like dermal macrophages are maintained via IL-4/CCL24–mediated cooperative interaction with eosinophils in cutaneous leishmaniasis
Science Immunology, Apr 9, 2020; DOI: 10.1126/sciimmunol.aaz4415
Oviductal sperm counting
Authors: Wipawee Winuthayanon and Carmen J Williams
Date: Apr 2, 2021，Views：844
Original protocol used in research article：
Oviductal estrogen receptor α signaling prevents protease-mediated embryo death
eLife, Dec 1, 2015; DOI: 10.7554/eLife.10453
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