Protocols shared by authors via RaP service
Journal
ChIP-seq
Author: Wen Tang
Date: Mar 15, 2021，Views：972
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
De novo protein design
Author: Anastassia A. Vorobieva
Date: Mar 15, 2021，Views：923
Original protocol used in research article：
De novo design of transmembrane β barrels
Science, Feb 18, 2021; DOI: 10.1126/science.abc8182
Organoid experiments culture & seeding
Authors: Wouter R. Karthaus, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：1032
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Prostate isolation and enzymatic digestion
Authors: Wouter R. Karthaus, Matan Hofree, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：1006
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Single-cell dissociation and RNA sequencing
Author: Michael L Wallace
Date: Mar 11, 2021，Views：931
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
Protein expression and purification
Authors: Claire Gorby and Ignacio Moraga
Date: Mar 11, 2021，Views：921
Original protocol used in research article：
Engineered IL-10 variants elicit potent immunomodulatory effects at low ligand doses
Science Signaling, Sep 14, 2020; DOI: 10.1126/scisignal.abc0653
Automated filopodia analysis software
Author: Johannes Stegmaier
Date: Mar 10, 2021，Views：963
Original protocol used in research article：
Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates
eLife, Jul 31, 2018; DOI: 10.7554/eLife.36953
Primary mouse EC isolation
Authors: Nora Yucel and Zoltan Arany
Date: Mar 10, 2021，Views：982
Original protocol used in research article：
Cardiac endothelial cells maintain open chromatin and expression of cardiomyocyte myofibrillar genes
eLife, Dec 14, 2020; DOI: 10.7554/eLife.55730
Imaging Cytosolic Calcium in T Lymphocytes: a protocol for use with genetically encoded or chemical Ca2+ indicators
Author: Michael D Cahalan
Date: Mar 10, 2021，Views：1193
Original protocol used in research article：
T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse
eLife, Dec 14, 2017; DOI: 10.7554/eLife.32417
Petri dish hygrotaxis arena
Author: Jennifer S Sun
Date: Mar 9, 2021，Views：896
Original protocol used in research article：
Humidity response depends on the small soluble protein Obp59a in Drosophila
eLife, Sep 19, 2018; DOI: 10.7554/eLife.39249
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