Protocols shared by authors via RaP service
Journal
Starvation of cells and re-stimulation with aa’s
Author: Maria Manifava
Date: Mar 1, 2021，Views：979
Original protocol used in research article：
Dynamics of mTORC1 activation in response to amino acids
eLife, Oct 11, 2016; DOI: 10.7554/eLife.19960
Wholemount in situ hybridization for spatial-temporal visualization of gene expression in mouse early post-implantation embryos
Authors: Xianfa Yang, Yingying Chen, Lu Song, Ting Zhang and Naihe Jing
Date: Feb 27, 2021，Views：1156
Original protocol used in research article：
The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways
eLife, Jun 14, 2014; DOI: 10.7554/eLife.02224
Fluorescent cell sorting from Adult Drosophila Brains
Authors: Dingbang Ma and Michael Rosbash
Date: Feb 26, 2021，Views：1074
Original protocol used in research article：
A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056
Sample correlation matrix
Author: Richard White
Date: Feb 26, 2021，Views：958
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
RNA-seq analysis
Authors: Laura A Lavery, Ying-Wooi Wan and Alexander Trostle
Date: Feb 26, 2021，Views：930
Original protocol used in research article：
Losing Dnmt3a dependent methylation in inhibitory neurons impairs neural function by a mechanism impacting Rett syndrome
eLife, Mar 11, 2020; DOI: 10.7554/eLife.52981
Isolation of thymic epithelial cells and thymocytes
Authors: Chris P Ponting and Georg A Holländer
Date: Feb 26, 2021，Views：1678
Original protocol used in research article：
Ageing compromises mouse thymus function and remodels epithelial cell differentiation
eLife, Aug 25, 2020; DOI: 10.7554/eLife.56221
Gene construction
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：927
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Antibody sequences
Authors: Yan Wu, Feng Gao and George F. Gao
Date: Feb 25, 2021，Views：962
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Cells and Viruses
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1065
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Protein expression and purification
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1536
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
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