Protocols shared by authors via RaP service
Journal
PIV analysis
Author: HaiYang Wang
Date: Mar 28, 2021，Views：1020
Original protocol used in research article：
Symmetry breaking in hydrodynamic forces drives meiotic spindle rotation in mammalian oocytes
Science Advances, Apr 3, 2020; DOI: 10.1126/sciadv.aaz5004
Spinning-disc confocal and 2P3A-DSLM imaging
Authors: Jia Zhao, Yanmei Liu and Liangyi Chen
Date: Mar 27, 2021，Views：1170
Original protocol used in research article：
In vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development
eLife, Jan 29, 2019; DOI: 10.7554/eLife.41540
Phylogenetic analysis of sleep-relevant genes
Authors: Junko Kusumi, Hiroyuki J. Kanaya and Taichi Q. Itoh
Date: Mar 27, 2021，Views：834
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Ce3D+ and Ce3D++ Tissue Clearing
Authors: Matthew J. Anderson, Valentin Magidson and Mark Lewandoski
Date: Mar 26, 2021，Views：1318
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
DNA binding assays
Author: Kyle W Muir
Date: Mar 26, 2021，Views：924
Original protocol used in research article：
Structural basis for Scc3-dependent cohesin recruitment to chromatin
eLife, Aug 15, 2018; DOI: 10.7554/eLife.38356
Hydroxylamine experiments
Authors: Luca Palazzo, Ivan Matic and Ivan Ahel
Date: Mar 26, 2021，Views：1133
Original protocol used in research article：
Serine is the major residue for ADP-ribosylation upon DNA damage
eLife, Feb 26, 2018; DOI: 10.7554/eLife.34334
Sucrose gradient fractionation
Author: Aleksra Filipovska
Date: Mar 25, 2021，Views：898
Original protocol used in research article：
Fidelity of translation initiation is required for coordinated respiratory complex assembly
Science Advances, Dec 19, 2019; DOI: 10.1126/sciadv.aay2118
Derivation of hTSCs from naïve hPSCs
Author: Chen Dong
Date: Mar 25, 2021，Views：1144
Original protocol used in research article：
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504
FAC sorting
Author: Noelia Urbán
Date: Mar 25, 2021，Views：928
Original protocol used in research article：
Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells
eLife, Sep 25, 2019; DOI: 10.7554/eLife.48561
Time-averaged restraints
Author: Tom Burnley
Date: Mar 25, 2021，Views：895
Original protocol used in research article：
Modelling dynamics in protein crystal structures by ensemble refinement
eLife, Dec 18, 2012; DOI: 10.7554/eLife.00311
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