Protocols shared by authors via RaP service
Journal
Reduced silver staining
Authors: Gabriella Hannah Wolff and Nicholas James Strausfeld
Date: Mar 18, 2021，Views：842
Original protocol used in research article：
An insect-like mushroom body in a crustacean brain
eLife, Sep 26, 2017; DOI: 10.7554/eLife.29889
Cell Clustering and cluster identification
Author: Michael L Wallace
Date: Mar 18, 2021，Views：840
Original protocol used in research article：
Anatomical and single-cell transcriptional profiling of the murine habenular complex
eLife, Feb 11, 2020; DOI: 10.7554/eLife.51271
First and second strand cDNA synthesis and pre-amplification
Author: Arthur M Talman
Date: Mar 18, 2021，Views：884
Original protocol used in research article：
Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites
eLife, Mar 27, 2018; DOI: 10.7554/eLife.33105
Generation of transgenic lines
Authors: Oriol Llorà-Batlle and Alfred Cortés
Date: Mar 17, 2021，Views：757
Original protocol used in research article：
Conditional expression of PfAP2-G for controlled massive sexual conversion in Plasmodium falciparum
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz5057
Isolation of T-cell subpopulations
Authors: Anastasia A Minervina and Mikhail V Pogorelyy
Date: Mar 17, 2021，Views：1347
Original protocol used in research article：
Primary and secondary anti-viral response captured by the dynamics and phenotype of individual T cell clones
eLife, Feb 21, 2020; DOI: 10.7554/eLife.53704
Strand-specific lncRNA sequencing and analysis
Authors: Shiqi Luo and Xin Zhou
Date: Mar 17, 2021，Views：875
Original protocol used in research article：
Gene reuse facilitates rapid radiation and independent adaptation to diverse habitats in the Asian honeybee
Science Advances, Dec 17, 2020; DOI: 10.1126/sciadv.abd3590
Generate dot surface of the channel hole for CALHM1 using HOLE program
Authors: Xue Yang and Yuequan Shen
Date: Mar 16, 2021，Views：906
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
MS/MS biclustering and molecular networking
Author: Dapeng Li
Date: Mar 16, 2021，Views：745
Original protocol used in research article：
Information theory tests critical predictions of plant defense theory for specialized metabolism
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz0381
Protein expression and purification
Author: Yue Ren
Date: Mar 16, 2021，Views：923
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
scVelo
Author: Bryan Marsh
Date: Mar 15, 2021，Views：2176
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
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