Protocols shared by authors via RaP service
Journal
Repair-Seq
Authors: Dylan A. Reid and Fred H. Gage
Date: Apr 12, 2021，Views：1151
Original protocol used in research article：
Incorporation of a nucleoside analog maps genome repair sites in postmitotic human neurons
Science, Apr 2, 2021; DOI: 10.1126/science.abb9032
Trend and attribution analysis
Authors: Ni Huang and Li Wang
Date: Apr 12, 2021，Views：911
Original protocol used in research article：
Spatial and temporal variations in global soil respiration and their relationships with climate and land cover
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb8508
Preparation of libraries for RNA-seq
Authors: Jamie R Wangen and Rachel Green
Date: Apr 9, 2021，Views：867
Original protocol used in research article：
Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides
eLife, Jan 23, 2020; DOI: 10.7554/eLife.52611
RRTS calculation
Authors: Jamie Wangen and Rachel Green
Date: Apr 9, 2021，Views：937
Original protocol used in research article：
Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides
eLife, Jan 23, 2020; DOI: 10.7554/eLife.52611
Bone sectioning, immunostaining, and confocal imaging
Authors: Lei Ding and Sean J Morrison
Date: Apr 9, 2021，Views：912
Original protocol used in research article：
Hematopoietic stem and progenitor cells regulate the regeneration of their niche by secreting Angiopoietin-1
eLife, Mar 30, 2015; DOI: 10.7554/eLife.05521
Statistical analysis
Authors: Thanaphong Phongpreecha and Thomas J. Montine
Date: Apr 8, 2021，Views：866
Original protocol used in research article：
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Contribution of inter-relatedness chimpanzees to differential expression analysis
Author: Benjamin Jung Fair
Date: Apr 8, 2021，Views：866
Original protocol used in research article：
Gene expression variability in human and chimpanzee populations share common determinants
eLife, Oct 21, 2020; DOI: 10.7554/eLife.59929
Single-nucleus RNA-seq data generation
Authors: Allen Wang, Kyle J Gaulton, Sebastian Preissl and Xin Sun
Date: Apr 7, 2021，Views：1063
Original protocol used in research article：
Single-cell multiomic profiling of human lungs reveals cell-type-specific and age-dynamic control of SARS-CoV2 host genes
eLife, Nov 9, 2020; DOI: 10.7554/eLife.62522
Microtubule co-sedimentation from worm extract
Author: Shaohe Wang
Date: Apr 7, 2021，Views：875
Original protocol used in research article：
NOCA-1 functions with γ-tubulin and in parallel to Patronin to assemble non-centrosomal microtubule arrays in C. elegans
eLife, Sep 15, 2015; DOI: 10.7554/eLife.08649
Extending 3' UTRs in the transcriptome index
Authors: Lauren M Saunders, Cole Trapnell and David M Parichy
Date: Apr 7, 2021，Views：1022
Original protocol used in research article：
Thyroid hormone regulates distinct paths to maturation in pigment cell lineages
eLife, May 29, 2019; DOI: 10.7554/eLife.45181
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