Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Lamas-Toranzo, I, Hamze, J, Jiménez-Movilla, M and Bermejo-Álvarez, P(2021). Protein extraction and immunobloting. Bio-protocol Preprint. bio-protocol.org/prep1071.
Lamas-Toranzo, I., Hamze, J. G., Bianchi, E., Fernández-Fuertes, B., Pérez-Cerezales, S., Laguna-Barraza, R., Fernández-González, R., Lonergan, P., Gutiérrez-Adán, A., Wright, G. J., Jiménez-Movilla, M. and Bermejo-Álvarez, P.(2020). TMEM95 is a sperm membrane protein essential for mammalian fertilization. eLIFE. DOI: 10.7554/eLife.53913