Protocols shared by authors via RaP service
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Generate dot surface of the channel hole for CALHM1 using HOLE program
Authors: Xue Yang and Yuequan Shen
Date: Mar 16, 2021，Views：941
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
MS/MS biclustering and molecular networking
Author: Dapeng Li
Date: Mar 16, 2021，Views：779
Original protocol used in research article：
Information theory tests critical predictions of plant defense theory for specialized metabolism
Science Advances, Jun 9, 2020; DOI: 10.1126/sciadv.aaz0381
Protein expression and purification
Author: Yue Ren
Date: Mar 16, 2021，Views：961
Original protocol used in research article：
Cryo-EM structure of the calcium homeostasis modulator 1 channel
Science Advances, Jul 16, 2020; DOI: 10.1126/sciadv.aba8161
scVelo
Author: Bryan Marsh
Date: Mar 15, 2021，Views：2293
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
Oil-Red-O staining to determine the amount of stored triglyceride in Caenorhabditis elegans
Authors: Avijit Mallick and Bhagwati P Gupta
Date: Mar 15, 2021，Views：889
Original protocol used in research article：
Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans
microPublication Biology, Jul 21, 2020; DOI: 10.17912/micropub.biology.000281
Calculation of VISTA module score
Author: Yanding Zhao
Date: Mar 15, 2021，Views：860
Original protocol used in research article：
VISTA is a checkpoint regulator for naïve T cell quiescence and peripheral tolerance
Science, Jan 16, 2020; DOI: 10.1126/science.aay0524
Virus neutralization assay
Authors: Yasumasa Iwatani and Hiroshi Murakami
Date: Mar 15, 2021，Views：982
Original protocol used in research article：
Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916
Computational methods
Authors: Lizhi Liu and Sheng Chen
Date: Mar 15, 2021，Views：844
Original protocol used in research article：
Mechanical penetration of β-lactam–resistant Gram-negative bacteria by programmable nanowires
Science Advances, Jul 2, 2020; DOI: 10.1126/sciadv.abb9593
Headloop PCR
Author: Gareth Thomas Powell
Date: Mar 15, 2021，Views：2479
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
ChIP-seq
Author: Wen Tang
Date: Mar 15, 2021，Views：972
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
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