In situ hybridization

Kaiwen Kam; Carolina Thörn Perez

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In situ hybridization

KK Kaiwen Kam

CT Carolina Thörn Perez

Last updated date: Apr 7, 2021 Views: 895 Forks: 0

An abbreviated version of this protocol was published in eLife in Dec, 2019

Opioids modulate an emergent rhythmogenic process to depress breathing

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Thank you Carolina for your message,

Here are the catalog numbers for the probes used.

Mm-Tac1 Cat No. 410351, Mm-Chat Cat No. 408731, Mm-Oprm1 Cat No. 315841

I noticed that in the quote I have, the Chat number is different (#410071, but I check with ACD, and they confirmed both are for the same probe).

You will have no issues adding a 3rd probe either with regular or v2 Assay. I used the protocol for fresh frozen tissues, and the user manual is available at: https://acdbio.com/documents/product-documents

Don't hesitate to contact me if needed. I recently moved to Canada!

Carolina Thörn Perez

How to cite：

Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:

Kam, K and Thörn Perez, C(2021). In situ hybridization. Bio-protocol Preprint. bio-protocol.org/prep999.
Sun, X., Thörn Pérez, C., Halemani D, N., Shao, X. M., Greenwood, M., Heath, S., Feldman, J. L. and Kam, K.(2019). Opioids modulate an emergent rhythmogenic process to depress breathing. eLife. DOI: 10.7554/eLife.50613