Protocols shared by authors via RaP service
Journal
Recovery cohousing following antibiotic treatment
Author: Aspen T Reese
Date: Mar 8, 2021，Views：883
Original protocol used in research article：
Antibiotic-induced changes in the microbiota disrupt redox dynamics in the gut
eLife, Jun 19, 2018; DOI: 10.7554/eLife.35987
Construction of plasmids
Author: Eric Klavins
Date: Mar 8, 2021，Views：981
Original protocol used in research article：
Synthetic hormone-responsive transcription factors can monitor and re-program plant development
eLife, May 1, 2018; DOI: 10.7554/eLife.34702
Protein expression and purification of the cysteine-rich domain of Frizzled receptors
Author: Tao-Hsin Chang
Date: Mar 7, 2021，Views：1099
Original protocol used in research article：
Structure and functional properties of Norrin mimic Wnt for signalling with Frizzled4, Lrp5/6, and proteoglycan
eLife, Jul 9, 2015; DOI: 10.7554/eLife.06554
Kmer mapping
Author: Bernadette C Young
Date: Mar 7, 2021，Views：1763
Original protocol used in research article：
Panton–Valentine leucocidin is the key determinant of Staphylococcus aureus pyomyositis in a bacterial GWAS
eLife, Feb 22, 2019; DOI: 10.7554/eLife.42486
Measurement of the labile iron pool
Author: Jumpei Ito
Date: Mar 5, 2021，Views：1649
Original protocol used in research article：
Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice
eLife, Feb 2, 2021; DOI: 10.7554/eLife.62174
crRNA selection
Authors: Francois Kroll and Jason Rihel
Date: Mar 5, 2021，Views：1299
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
Information flow analysis
Author: Lucie Delemotte
Date: Mar 4, 2021，Views：789
Original protocol used in research article：
Calmodulin acts as a state-dependent switch to control a cardiac potassium channel opening
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abd6798
Lineage Labeling & Cell Transfer
Author: Bruce B Riley
Date: Mar 4, 2021，Views：848
Original protocol used in research article：
The Warburg Effect and lactate signaling augment Fgf-MAPK to promote sensory-neural development in the otic vesicle
eLife, Apr 27, 2020; DOI: 10.7554/eLife.56301
Data collection
Author: Melody G Campbell
Date: Mar 4, 2021，Views：1020
Original protocol used in research article：
2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
eLife, Mar 11, 2015; DOI: 10.7554/eLife.06380
ATAC-seq protocol
Authors: Haoze Vincent Yu and Neil Segil
Date: Mar 4, 2021，Views：959
Original protocol used in research article：
Foxc1 establishes enhancer accessibility for craniofacial cartilage differentiation
eLife, Jan 27, 2021; DOI: 10.7554/eLife.63595
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