Protocols shared by authors via RaP service
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Assembly, transformation, and selection of the IL-6 library
Authors: Jonathan Martinez-Fabregas, Elizabeth Pohler and Ignacio Moraga
Date: Mar 2, 2021，Views：893
Original protocol used in research article：
Kinetics of cytokine receptor trafficking determine signaling and functional selectivity
eLife, Nov 27, 2019; DOI: 10.7554/eLife.49314
Affinity purification and mass spectrometry analysis
Author: Mathieu Rougemaille
Date: Mar 2, 2021，Views：849
Original protocol used in research article：
Ubiquitination-dependent control of sexual differentiation in fission yeast
eLife, Aug 25, 2017; DOI: 10.7554/eLife.28046
Preparation of XaxAB pore complexes
Author: Evelyn Schubert
Date: Mar 2, 2021，Views：922
Original protocol used in research article：
Membrane insertion of α-xenorhabdolysin in near-atomic detail
eLife, Jul 16, 2018; DOI: 10.7554/eLife.38017
Read data processing
Author: Maximilian R Stammnitz
Date: Mar 2, 2021，Views：1004
Original protocol used in research article：
Freshwater monitoring by nanopore sequencing
eLife, Jan 19, 2021; DOI: 10.7554/eLife.61504
DNA extraction
Author: Maximilian R Stammnitz
Date: Mar 2, 2021，Views：1252
Original protocol used in research article：
Freshwater monitoring by nanopore sequencing
eLife, Jan 19, 2021; DOI: 10.7554/eLife.61504
SAM and SAH quantification
Author: Carolina Magdalen Greco
Date: Mar 1, 2021，Views：818
Original protocol used in research article：
S-adenosyl-l-homocysteine hydrolase links methionine metabolism to the circadian clock and chromatin remodeling
Science Advances, Dec 15, 2020; DOI: 10.1126/sciadv.abc5629
CRISPR library construction and screening
Authors: Rui Lu and Chunliang Li
Date: Mar 1, 2021，Views：1181
Original protocol used in research article：
Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen
eLife, Oct 1, 2020; DOI: 10.7554/eLife.57858
Starvation of cells and re-stimulation with aa’s
Author: Maria Manifava
Date: Mar 1, 2021，Views：1067
Original protocol used in research article：
Dynamics of mTORC1 activation in response to amino acids
eLife, Oct 11, 2016; DOI: 10.7554/eLife.19960
Wholemount in situ hybridization for spatial-temporal visualization of gene expression in mouse early post-implantation embryos
Authors: Xianfa Yang, Yingying Chen, Lu Song, Ting Zhang and Naihe Jing
Date: Feb 27, 2021，Views：1199
Original protocol used in research article：
The transcription factor Pou3f1 promotes neural fate commitment via activation of neural lineage genes and inhibition of external signaling pathways
eLife, Jun 14, 2014; DOI: 10.7554/eLife.02224
Fluorescent cell sorting from Adult Drosophila Brains
Authors: Dingbang Ma and Michael Rosbash
Date: Feb 26, 2021，Views：1108
Original protocol used in research article：
A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056
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