Protocols shared by authors via RaP service
Journal
scVelo
Author: Bryan Marsh
Date: Mar 15, 2021，Views：2518
Original protocol used in research article：
Single nuclei RNA-seq of mouse placental labyrinth development
eLife, Nov 3, 2020; DOI: 10.7554/eLife.60266
Oil-Red-O staining to determine the amount of stored triglyceride in Caenorhabditis elegans
Authors: Avijit Mallick and Bhagwati P Gupta
Date: Mar 15, 2021，Views：1456
Original protocol used in research article：
Vitellogenin-2 acts downstream of PRY-1/Axin to regulate lipids and lifespan in C. elegans
microPublication Biology, Jul 21, 2020; DOI: 10.17912/micropub.biology.000281
Calculation of VISTA module score
Author: Yanding Zhao
Date: Mar 15, 2021，Views：951
Original protocol used in research article：
VISTA is a checkpoint regulator for naïve T cell quiescence and peripheral tolerance
Science, Jan 16, 2020; DOI: 10.1126/science.aay0524
Virus neutralization assay
Authors: Yasumasa Iwatani and Hiroshi Murakami
Date: Mar 15, 2021，Views：1081
Original protocol used in research article：
Antibody-like proteins that capture and neutralize SARS-CoV-2
Science Advances, Oct 14, 2020; DOI: 10.1126/sciadv.abd3916
Computational methods
Authors: Lizhi Liu and Sheng Chen
Date: Mar 15, 2021，Views：940
Original protocol used in research article：
Mechanical penetration of β-lactam–resistant Gram-negative bacteria by programmable nanowires
Science Advances, Jul 2, 2020; DOI: 10.1126/sciadv.abb9593
Headloop PCR
Author: Gareth Thomas Powell
Date: Mar 15, 2021，Views：2868
Original protocol used in research article：
A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes
eLife, Jan 8, 2021; DOI: 10.7554/eLife.59683
ChIP-seq
Author: Wen Tang
Date: Mar 15, 2021，Views：1098
Original protocol used in research article：
ESCO1 and CTCF enable formation of long chromatin loops by protecting cohesinSTAG1 from WAPL
eLife, Feb 17, 2020; DOI: 10.7554/eLife.52091
De novo protein design
Author: Anastassia A. Vorobieva
Date: Mar 15, 2021，Views：1025
Original protocol used in research article：
De novo design of transmembrane β barrels
Science, Feb 18, 2021; DOI: 10.1126/science.abc8182
Organoid experiments culture & seeding
Authors: Wouter R. Karthaus, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：1117
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
Prostate isolation and enzymatic digestion
Authors: Wouter R. Karthaus, Matan Hofree, Aviv Regev and Charles L. Sawyers
Date: Mar 13, 2021，Views：1100
Original protocol used in research article：
Regenerative potential of prostate luminal cells revealed by single-cell analysis
Science, Apr 30, 2020; DOI: 10.1126/science.aay0267
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