Protocols shared by authors via RaP service
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Identification of sex-differential gene expression
Authors: Meritxell Oliva, Barbara E. Stranger, Manuel Muñoz-Aguirre and Yuxin Zou
Date: Feb 2, 2021，Views：968
Original protocol used in research article：
The impact of sex on gene expression across human tissues
Science, Sep 11, 2020; DOI: 10.1126/science.aba3066
TCR analysis
Author: Paul Gueguen
Date: Feb 2, 2021，Views：880
Original protocol used in research article：
Contribution of resident and circulating precursors to tumor-infiltrating CD8+ T cell populations in lung cancer
Science Immunology, Jan 29, 2021; DOI: 10.1126/sciimmunol.abd5778
Structural determination and refinement
Author: Ming quan Xie
Date: Feb 2, 2021，Views：779
Original protocol used in research article：
Structural insights into the signal transduction mechanism of the K+-sensing two-component system KdpDE
Science Signaling, Aug 3, 2020; DOI: 10.1126/scisignal.aaz2970
Modulation of RBC cholesterol
Author: Rima S Chakrabarti
Date: Feb 1, 2021，Views：823
Original protocol used in research article：
Variability of cholesterol accessibility in human red blood cells measured using a bacterial cholesterol-binding toxin
eLife, Feb 8, 2017; DOI: 10.7554/eLife.23355
Association between the Wuhan city travel ban and the arrival time of COVID-19 in other cities
Authors: Yonghong Liu and Chieh-Hsi Wu
Date: Feb 1, 2021，Views：983
Original protocol used in research article：
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
Science, May 7, 2020; DOI: 10.1126/science.abb6105
Fc.IL-2 muteins
Authors: Daniel J. Campbell and Marc A. Gavin
Date: Feb 1, 2021，Views：932
Original protocol used in research article：
An IL-2 mutein engineered to promote expansion of regulatory T cells arrests ongoing autoimmunity in mice
Science Immunology, Aug 13, 2020; DOI: 10.1126/sciimmunol.aba5264
Ion transport assay of SzRs in E. coli cells
Authors: Masae Konno, Keiichi Inoue and Hideki Kandori
Date: Jan 31, 2021，Views：4539
Original protocol used in research article：
Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps
Science Advances, Apr 9, 2020; DOI: 10.1126/sciadv.aaz2441
Immunofluorescence staining of heart frozen sections
Author: Huang-Tian Yang
Date: Jan 31, 2021，Views：985
Original protocol used in research article：
Strontium ions protect hearts against myocardial ischemia/reperfusion injury
Science Advances, Jan 15, 2021; DOI: 10.1126/sciadv.abe0726
ATAC-seq significance analysis
Author: Wen Zhang
Date: Jan 31, 2021，Views：914
Original protocol used in research article：
Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC-derived retinal organoids
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay5247
Western blotting
Authors: Jie Yang and Yuan Wang
Date: Jan 30, 2021，Views：903
Original protocol used in research article：
Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation leading to impaired CD8+ T cell response in tumor
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba5412
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