Protocols shared by authors via RaP service
Journal
HCN4 immunostaining in human (Alomone labs antibody)
Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021，Views：851
Original protocol used in research article：
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492
Migration assay
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 12, 2021，Views：945
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Generation of in vitro trained TAMs
Authors: Poonam Ashwin Kumar, Andreas Weigert and Rajkumar Savai
Date: Feb 12, 2021，Views：955
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Article inclusion
Author: Alyson Haslam
Date: Feb 12, 2021，Views：1086
Original protocol used in research article：
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183
Data analysis
Author: Alyson Haslam
Date: Feb 12, 2021，Views：1104
Original protocol used in research article：
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183
Flow cytometry
Authors: Takuya Sekine, André Perez-Potti and Marcus Buggert
Date: Feb 11, 2021，Views：995
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Clinical Trial Design: Summary, Recipient Eligibility Criteria, Donor Selection Criteria, Donor/Recipient Seromatching, and Study Conduct
Author: Diwakar Davar
Date: Feb 11, 2021，Views：984
Original protocol used in research article：
Fecal microbiota transplant overcomes resistance to anti–PD-1 therapy in melanoma patients
Science, Feb 5, 2021; DOI: 10.1126/science.abf3363
Dimensionality reduction and clustering
Authors: Dingbang Ma, Dariusz Przybylski, Katharine Abruzzi, Matthias Schlichting, Qunlong Li, Xi Long and Michael Rosbash
Date: Feb 11, 2021，Views：1114
Original protocol used in research article：
A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056
Single-cell RNA library preparation
Authors: Dingbang Ma, Dariusz Przybylski, Katharine Abruzzi, Matthias Schlichting, Qunlong Li, Xi Long and Michael Rosbash
Date: Feb 11, 2021，Views：1148
Original protocol used in research article：
A transcriptomic taxonomy of Drosophila circadian neurons around the clock
eLife, Jan 13, 2021; DOI: 10.7554/eLife.63056
Protocol of full article
Author: Shakil Ahmed
Date: Feb 10, 2021，Views：935
Original protocol used in research article：
Pyrogallol induces oxidative stress defects in the fission yeast S. pombe
microPublication Biology, Jan 7, 2021; DOI: 10.17912/micropub.biology.000348
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