Protocols shared by authors via RaP service
Journal
Assessment of thrombolysis efficacy in a mouse model
Authors: Yu Gao and Wang si yu
Date: Feb 3, 2021，Views：836
Original protocol used in research article：
Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204
Immunohistochemistry protocol for analyzing Hedgehog pathway factors in mouse embryos
Authors: Saishu Yoshida and Kiyotsugu Yoshida
Date: Feb 3, 2021，Views：881
Original protocol used in research article：
The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
eLife, Aug 6, 2020; DOI: 10.7554/eLife.57381
tRNA charging assay
Authors: Natalya N Pavlova and Craig B Thompson
Date: Feb 3, 2021，Views：1657
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Global protein synthesis quantification in Caenorhabditis elegans by puromycin incorporation
Authors: Clemens Heissenberger and Markus Schosserer
Date: Feb 3, 2021，Views：1083
Original protocol used in research article：
The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans
eLife, Dec 8, 2020; DOI: 10.7554/eLife.56205
Sperm motility
Authors: Sylvia Fechner, U Benjamin Kaupp and Reinhard Seifert
Date: Feb 3, 2021，Views：851
Original protocol used in research article：
A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm
eLife, Dec 9, 2015; DOI: 10.7554/eLife.07624
Animal immunization and challenge studies
Authors: Shibo Jiang, Shihui Sun and Cheng-Feng Qin
Date: Feb 3, 2021，Views：941
Original protocol used in research article：
Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
Science, Sep 24, 2020; DOI: 10.1126/science.abc4730
Construction, expression, and purification of recombinant RBD-Fc.
Authors: cheng-feng Qin, shihui sun and shibo jiang
Date: Feb 3, 2021，Views：885
Original protocol used in research article：
Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
Science, Sep 24, 2020; DOI: 10.1126/science.abc4730
Lifespan assay
Authors: Jongsun Lee, Sieun S. and Seung-Jae V.
Date: Feb 2, 2021，Views：781
Original protocol used in research article：
A mutation that alters the 255th glutamate to lysine in RSKS-1/S6 kinase reliably extends the lifespan of C. elegans
microPublication Biology, Oct 27, 2020; DOI: 10.17912/micropub.biology.000322
Dissection
Authors: Vera D Rinaldi and Oliver J Rando
Date: Feb 2, 2021，Views：872
Original protocol used in research article：
An atlas of cell types in the mouse epididymis and vas deferens
eLife, Jul 30, 2020; DOI: 10.7554/eLife.55474
Gene set enrichment analysis (GSEA) of sex-biased genes
Authors: Meritxell Oliva, Barbara E. Stranger and Daniel Cotter
Date: Feb 2, 2021，Views：980
Original protocol used in research article：
The impact of sex on gene expression across human tissues
Science, Sep 11, 2020; DOI: 10.1126/science.aba3066
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