Protocols shared by authors via RaP service
Journal
mRNAs half-lives determination
Author: Ning Dai
Date: Feb 16, 2021，Views：848
Original protocol used in research article：
IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1
eLife, Jul 28, 2017; DOI: 10.7554/eLife.27155
Protein expression, purification, and site-specific labeling
Author: Jesper Levring
Date: Feb 16, 2021，Views：975
Original protocol used in research article：
Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses
eLife, May 27, 2020; DOI: 10.7554/eLife.56451
Cloning of mlCNBD-FRET
Author: Dagmar Wachten
Date: Feb 16, 2021，Views：962
Original protocol used in research article：
A novel biosensor to study cAMP dynamics in cilia and flagella
eLife, Mar 22, 2016; DOI: 10.7554/eLife.14052
Muscle myoblast isolation and culture
Author: Shihuan Kuang
Date: Feb 15, 2021，Views：921
Original protocol used in research article：
Stage-specific effects of Notch activation during skeletal myogenesis
eLife, Sep 19, 2016; DOI: 10.7554/eLife.17355
Multiplex FISH in CaRMA-imaged tissue after in vivo calcium imaging
Authors: Hui Yang, Shengjin Xu and Scott M. Sternson
Date: Feb 15, 2021，Views：978
Original protocol used in research article：
Behavioral state coding by molecularly defined paraventricular hypothalamic cell type ensembles
Science, Oct 15, 2020; DOI: 10.1126/science.abb2494
Alignment of the GRIN lens
Authors: Zhongya Qin, Nancy Y. Ip and Jianan Y. Qu
Date: Feb 15, 2021，Views：979
Original protocol used in research article：
Adaptive optics two-photon endomicroscopy enables deep-brain imaging at synaptic resolution over large volumes
Science Advances, Sep 29, 2020; DOI: 10.1126/sciadv.abc6521
HCN4 immunostaining in human (Alomone labs antibody)
Authors: Angélique Bordey and Lena Nguyen
Date: Feb 13, 2021，Views：818
Original protocol used in research article：
Ectopic HCN4 expression drives mTOR-dependent epilepsy in mice
Science Translational Medicine, Nov 17, 2020; DOI: 10.1126/scitranslmed.abc1492
Migration assay
Authors: Poonam Ashwin Kumar Sarode and Rajkumar Savai
Date: Feb 12, 2021，Views：903
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Generation of in vitro trained TAMs
Authors: Poonam Ashwin Kumar, Andreas Weigert and Rajkumar Savai
Date: Feb 12, 2021，Views：922
Original protocol used in research article：
Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer
Science Advances, Jun 4, 2020; DOI: 10.1126/sciadv.aaz6105
Article inclusion
Author: Alyson Haslam
Date: Feb 12, 2021，Views：1035
Original protocol used in research article：
A comprehensive review of randomized clinical trials in three medical journals reveals 396 medical reversals
eLife, Jun 11, 2019; DOI: 10.7554/eLife.45183
Other Resources
www.bio-protocol.org
for advanced protocols
www.bio-thing.com
for reagents & equipment
lab.bio-thing.cn
for lab management
For Advertisers
Advertising Integrity
Board
Advertising Policy
Advertising Terms and
Conditions
For Authors
Submission Procedure
Author guidelines
Submit a Protocol
About
About Us

Become an Ambassador
©  Bio-protocol LLC. ISSN: 2331-8325 京ICP备11029730号-1 Terms of Service | Privacy Policy | Contact Us
Find out more
We use cookies to improve your user experience on this site. By using our website, you agree to the storage of cookies on your computer.