Mafalda Escobar-Henriques University of Cologne Cologne
2 protocols

Ira Buntenbroich University of Cologne Cologne
1 protocol

Fabian den Brave Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Germany, Germany,
1 protocol

Ramona Schuster Institute for Genetics, Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases, University of Cologne, Germany, Germany,
1 protocol

Tânia Simões
  • University of Cologne Cologne
  • 2 Author merit


PhD, Universidade Técnica de Lisboa, Portugal, 2006

Current position

Pos-doc, CECAD, Genetics Institute, University of Cologne, Germany


  1. Simões T, Schuster R, den Brave F, Escobar-Henriques M. (2018) Cdc48 regulates a deubiquitylase cascade critical for mitochondrial fusion. Elife: e30015, doi: 10.7554/eLife.30015.
  2. Vicente Miranda H, Szego ÉM, Oliveira LMA, Breda C, Darendelioglu E, de Oliveira RM, Ferreira DG, Gomes MA, Rott R, Oliveira M, Munari F, Enguita FJ, Simões T, Rodrigues EF, Heinrich M, Martins IC, Zamolo I, Riess O, Cordeiro C, Ponces-Freire A, Lashuel HA, Santos NC, Lopes LV, Xiang W, Jovin TM, Penque D, Engelender S, Zweckstetter M, Klucken J, Giorgini F, Quintas A, Outeiro TF. (2017) Glycation potentiates α-synuclein-associated neurodegeneration in synucleinopathies. Brain.140(5):1399-1419
  3. Vicente Miranda H, Xiang W, de Oliveira RM, Simões T, Pimentel J, Klucken J, Penque D, Outeiro TF. (2013) Heat-mediated enrichment of α-synuclein from cells and tissue for assessing post-translational modifications. J Neurochem. 126(5):673-84.
  4. Sebastiana M, Figueiredo A, Monteiro F, Martins J, Franco C, Coelho AV, Vaz F, Simões T, Penque D, Pais MS, Ferreira S. (2013) A possible approach for gel-based proteomic studies in recalcitrant woody plants. Springerplus. 2(1):210.
  5. Pacheco SA, Torres VM, Louro H, Gomes F, Lopes C, Marçal N, Fragoso E, Martins C, Oliveira CL, Hagenfeldt M, Bugalho-Almeida A, Penque D and Simões T.(2013) Effects of occupational exposure to tobacco smoke: Is there a link between environmental exposure and disease? J Toxicol Environ Health A. 76(4-5):311-27.
  6. Pacheco SA., Aguiar F, Ruivo P, Proença MC, Sekera M, Penque D, Simões T (2012) Occupational exposure to environmental tobacco smoke: a study in Lisbon restaurants. J Toxicol Environ Health A. 75(13-15):857-66.
  7. Simões T, Charro N, Alexandre Bruno M., Penque D. (2012) Proteomics in detection and monitoring chronic lung diseases: the human nasal epithelium as a molecular model. J Aller Ther S7:004. 
  8. Simões T, Charro N, Blonder J, Faria D, Couto FM, Chan KC, Waybright T, Isaaq HJ, Veenstra TD, Penque D. (2011) Molecular profiling of the human nasal epithelium: A proteomics approach. J Proteomics 75(1):56-69. 
  9. Penque D, Simões T, Amado F. (2011) Proteomics advances in the last decade: What is next? J Proteomics 75(1):1-3.
  10. Cox J, M A Heeren R, James P, Jorrin-Novo JV, Kolker E, Levander F, Morrice N, Picotti P, Righetti PG, Sánchez JC, Turck CW, Zubarev R, Alexandre BM, Corrales FJ, Marko-Varga G, O'Donovan S, O'Neil S, Prechl J, Simões T, Weckwerth W, Penque D. (2011) Facing challenges in Proteomics today and in the coming decade: Report of Roundtable Discussions at the 4th EuPA Scientific Meeting, Portugal, Estoril 2010. J Proteomics 75(1):4-17. 
  11. Louro H., Antunes S., Vital N., Gomes F., Penque D., Simões T., Silva M.J. (2011) Exposure to second-hand smoke in occupational settings: biomarkers of DNA damage and susceptibility. Mutagenesis, 26: pag.701-702.
  12. Santos PM, Simões T e Sá-Correia I (2009) Insights into yeast adaptive response to the agricultural fungicide mancozeb: A toxicoproteomics approach. Proteomics 9:657-70.
  13. Teixeira MC, Dias PJ, Simões T, Sá-Correia I. (2008) Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1. Biochem Biophys Res Commun 367: 249-55.
  14. Simões T, Mira NP, Fernandes AR, Sá-Correia I (2006) The SPI1 gene, encoding a glycosylphosphatidylinositol (GPI)-cell wall protein, plays a prominent role in the development of yeast resistance to lipophilic weak acids food preservatives. Appl Environ Microbiol, 72: 7168-75.
  15. Simões T, Teixeira MC, Fernandes AR, Sá-Correia I (2003) Saccharomyces cerevisiae adaptation to the herbicide 2,4-dichlorophenoxyacetic acid mediated by Msn2p/Msn4p-regulated genes: role of SPI1. Appl Environ Microbiol 69: 4019-28.
  16. Simões T, Basem MA,Fernandes AR, Strauss J, Seiboth B, Sá-Correia I e Kubicek CP (2000) Glucose does not activate the plasma-membrane-bound-H+-ATPase but affects pmaA transcript abundance in Aspergillus nidulans. Arch Microbiol 174: 340-345.
    4th EuPA Scientific Meeting - A proteomics Odyssey Towards Next Decades. Estoril, PORTUGAL. October 23-27, 2010. Editors: Gyorgy Marko-Varga & Tânia Simões. ISBN 978-963-86156-5-7
3 Protocols published
Analysis of Protein Stability by Synthesis Shutoff
Authors:  Ira Buntenbroich, Tânia Simões and Mafalda Escobar-Henriques, date: 11/20/2021, view: 1795, Q&A: 0

In this protocol, we describe the analysis of protein stability over time, using synthesis shutoff. As an example, we express HA-tagged yeast mitofusin Fzo1 in Saccharomyces cerevisiae and inhibit translation via cycloheximide (CHX). Proteasomal

Separation and Visualization of Low Abundant Ubiquitylated Forms
Authors:  Ramona Schuster, Tânia Simões, Fabian den Brave and Mafalda Escobar-Henriques, date: 11/20/2018, view: 4374, Q&A: 0
In this protocol we describe the separation and visualization of ubiquitylated forms of the yeast mitofusin Fzo1 by Western blot. To this aim, we express HA-tagged Fzo1 in Saccharomyces cerevisiae, break the cells to extract a ...
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