Improve Research Reproducibility A Bio-protocol resource
bpdetail_icon_lock
Preprint

Cross-temporal decoding

CT Cheng Tang

Last updated date: Mar 22, 2021 Views: 965 Forks: 0

original An abbreviated version of this protocol was published in eLife in Sep, 2020
Minimally dependent activity subspaces for working memory and motor preparation in the lateral prefrontal cortex
download pdf Download PDF
ask Ask a question
cite How to cite
nfavorite Favorite
  1. Baseline-subtract all the cells' firing rate (baseline is 300ms after fixation and before target onset).
  2. Split each cell's trials into the training set and the testing set (half and half, random)
  3. Construct pseudo-trials from training set and testing set for later discrimination analysis. (An example pseudo-trial from the training set: for all N neurons, randomly pick one trial from each cell's training set, so this pseudo-trial contains data from all N neurons as if they were recorded simultaneously.) For each target location, we constructed 1000 pseudo-trials. In total, we had 1000x7 training trials and 1000x7 testing trials.
  4. For each training time point and each testing time point, we performed PCA to reduce the dimensionality of the training data to explain 95% of the original variance (denoise the data), and projected the testing data onto the same PCs.
  5. For each training time point and each testing time point, an LDA classifier (linear discrimination analysis, MATLAB function classify) was trained on the denoised training data and tested on the denoised testing data and the performance was reported. 
Copyright: Content may be subjected to copyright.
How to cite：
Readers should cite both the Bio-protocol preprint and the original research article where this protocol was used:
  1. Tang, C(2021). Cross-temporal decoding. Bio-protocol Preprint. bio-protocol.org/prep955.
  2. Tang, C., Herikstad, R., Parthasarathy, A., Libedinsky, C. and Yen, S.(2020). Minimally dependent activity subspaces for working memory and motor preparation in the lateral prefrontal cortex. eLife. DOI: 10.7554/eLife.58154

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A
This protocol preprint was submitted via the "Request a Protocol" track.