Protocols shared by authors via RaP service
Journal
Barcode to gRNA assignment
Authors: Ryan Muller and Nicholas T. Ingolia
Date: Jan 27, 2021，Views：846
Original protocol used in research article：
CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes
Science, Dec 11, 2020; DOI: 10.1126/science.abb9662
Cryo-EM structural analysis of Ad5-3M
Author: Phoebe L. Stewart
Date: Jan 27, 2021，Views：925
Original protocol used in research article：
Systemic cancer therapy with engineered adenovirus that evades innate immunity
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.abc6659
Creating acetic acid-induced artifical gastric ulcers in rats
Authors: Philip Wai-Yan Chiu, Liming Bian and Xiayi Xu
Date: Jan 27, 2021，Views：859
Original protocol used in research article：
Bioadhesive hydrogels demonstrating pH-independent and ultrafast gelation promote gastric ulcer healing in pigs
Science Translational Medicine, Aug 25, 2020; DOI: 10.1126/scitranslmed.aba8014
Plasmids
Author: Seokhwi Kim
Date: Jan 26, 2021，Views：757
Original protocol used in research article：
Dynamic Fas signaling network regulates neural stem cell proliferation and memory enhancement
Science Advances, Apr 21, 2020; DOI: 10.1126/sciadv.aaz9691
Concordance and differential pathway analysis
Author: Alvin Shi
Date: Jan 26, 2021，Views：775
Original protocol used in research article：
Plasma-derived extracellular vesicle analysis and deconvolution enable prediction and tracking of melanoma checkpoint blockade outcome
Science Advances, Nov 12, 2020; DOI: 10.1126/sciadv.abb3461
mRNA synthesis and formulation
Author: John L. Joyal
Date: Jan 26, 2021，Views：1183
Original protocol used in research article：
Impact of mRNA chemistry and manufacturing process on innate immune activation
Science Advances, Jun 23, 2020; DOI: 10.1126/sciadv.aaz6893
CAT filter assay
Author: Aurélien Olichon
Date: Jan 26, 2021，Views：944
Original protocol used in research article：
NaLi-H1: A universal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
eLife, Jul 19, 2016; DOI: 10.7554/eLife.16228
Combined phosphoflow with RNA flow cytometry
Authors: Claire Ma, John C Marioni, Arianne C Richard and Gillian M Griffiths
Date: Jan 25, 2021，Views：1012
Original protocol used in research article：
Stimulation strength controls the rate of initiation but not the molecular organisation of TCR-induced signalling
eLife, May 15, 2020; DOI: 10.7554/eLife.53948
Whole-mount in situ hybridization of Hydra
Authors: Hiroyuki J. Kanaya, Yoshitaka Kobayakawa and Taichi Q. Itoh
Date: Jan 24, 2021，Views：938
Original protocol used in research article：
A sleep-like state in Hydra unravels conserved sleep mechanisms during the evolutionary development of the central nervous system
Science Advances, Oct 6, 2020; DOI: 10.1126/sciadv.abb9415
Differential gene expression analysis of bulk RNA-seq data
Author: Mohamed Nadhir Djekidel
Date: Jan 24, 2021，Views：938
Original protocol used in research article：
A transcriptional roadmap for 2C-like–to–pluripotent state transition
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aay5181
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