Protocols shared by authors via RaP service
Journal
Detailed reprogramming protocol
Author: Avani Yeola
Date: Feb 4, 2021，Views：896
Original protocol used in research article：
Induction of muscle-regenerative multipotent stem cells from human adipocytes by PDGF-AB and 5-azacytidine
Science Advances, Jan 13, 2021; DOI: 10.1126/sciadv.abd1929
Animal Study
Author: Thomas F. Rogers
Date: Feb 4, 2021，Views：922
Original protocol used in research article：
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model
Science, Aug 21, 2020; DOI: 10.1126/science.abc7520
Exploratory bifactor analysis
Authors: Christopher Gagne and Sonia J Bishop
Date: Feb 4, 2021，Views：1007
Original protocol used in research article：
Impaired adaptation of learning to contingency volatility in internalizing psychopathology
eLife, Dec 22, 2020; DOI: 10.7554/eLife.61387
SUMOylation and in vitro SUMOylation assay
Author: Xiancai Ma
Date: Feb 4, 2021，Views：896
Original protocol used in research article：
TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42426
Assessment of thrombolysis efficacy in a mouse model
Authors: Yu Gao and Wang si yu
Date: Feb 3, 2021，Views：869
Original protocol used in research article：
Accelerating thrombolysis using a precision and clot-penetrating drug delivery strategy by nanoparticle-shelled microbubbles
Science Advances, Jul 29, 2020; DOI: 10.1126/sciadv.aaz8204
Immunohistochemistry protocol for analyzing Hedgehog pathway factors in mouse embryos
Authors: Saishu Yoshida and Kiyotsugu Yoshida
Date: Feb 3, 2021，Views：924
Original protocol used in research article：
The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis
eLife, Aug 6, 2020; DOI: 10.7554/eLife.57381
tRNA charging assay
Authors: Natalya N Pavlova and Craig B Thompson
Date: Feb 3, 2021，Views：1780
Original protocol used in research article：
Translation in amino-acid-poor environments is limited by tRNAGln charging
eLife, Dec 8, 2020; DOI: 10.7554/eLife.62307
Global protein synthesis quantification in Caenorhabditis elegans by puromycin incorporation
Authors: Clemens Heissenberger and Markus Schosserer
Date: Feb 3, 2021，Views：1151
Original protocol used in research article：
The ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans
eLife, Dec 8, 2020; DOI: 10.7554/eLife.56205
Sperm motility
Authors: Sylvia Fechner, U Benjamin Kaupp and Reinhard Seifert
Date: Feb 3, 2021，Views：883
Original protocol used in research article：
A K+-selective CNG channel orchestrates Ca2+ signalling in zebrafish sperm
eLife, Dec 9, 2015; DOI: 10.7554/eLife.07624
Animal immunization and challenge studies
Authors: Shibo Jiang, Shihui Sun and Cheng-Feng Qin
Date: Feb 3, 2021，Views：980
Original protocol used in research article：
Adaptation of SARS-CoV-2 in BALB/c mice for testing vaccine efficacy
Science, Sep 24, 2020; DOI: 10.1126/science.abc4730
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