Isolation of thymic epithelial cells and thymocytes

Lymphocytes were released from thymic lobes,  via mechanical disruption between frosted glass slides (Thermo Scientific). Cells were then washed with ice-cold FACS buffer and centrifuged at 1500rpm for 5 minutes at 4°C. Cell pellets were resuspended in FACS buffer, filtered through a 70µm cell strainer (Greiner Bio-One) and counted before being prepared for analysis and or isolation via Flow Cytometry.
Thymic cellularity of a 4 week old female C57BL/6 is  ~ 400 Million cells.

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