Protocols shared by authors via RaP service
Journal
Protein expression and purification
Authors: Yan Wu, George Fu Gao and Feng Gao
Date: Feb 25, 2021，Views：1690
Original protocol used in research article：
A noncompeting pair of human neutralizing antibodies block COVID-19 virus binding to its receptor ACE2
Science, Jun 11, 2020; DOI: 10.1126/science.abc2241
Particle tracking
Author: Masazumi Fujiwara
Date: Feb 25, 2021，Views：1384
Original protocol used in research article：
Real-time nanodiamond thermometry probing in vivo thermogenic responses
Science Advances, Sep 11, 2020; DOI: 10.1126/sciadv.aba9636
ZFA enrichment
Author: Richard J White
Date: Feb 25, 2021，Views：1211
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
Plasmid constructs
Author: Theodore S. Jardetzky
Date: Feb 24, 2021，Views：979
Original protocol used in research article：
EphrinB2 clustering by Nipah virus G is required to activate and trap F intermediates at supported lipid bilayer–cell interfaces
Science Advances, Jan 27, 2021; DOI: 10.1126/sciadv.abe1235
List of antibodies, siRNAs and shRNAs
Author: Seyed Mehdi Jafarnejad
Date: Feb 24, 2021，Views：1044
Original protocol used in research article：
Translational control of ERK signaling through miRNA/4EHP-directed silencing
eLife, Feb 7, 2018; DOI: 10.7554/eLife.35034
RNA extraction from individual zebrafish embryos
Authors: Neha Wali, Richard White and Elisabeth M Busch-Nentwich
Date: Feb 24, 2021，Views：1596
Original protocol used in research article：
A high-resolution mRNA expression time course of embryonic development in zebrafish
eLife, Nov 16, 2017; DOI: 10.7554/eLife.30860
Cell culture, infection and vitrification
Authors: Georg Wolff and Montserrat Bárcena
Date: Feb 24, 2021，Views：1296
Original protocol used in research article：
A molecular pore spans the double membrane of the coronavirus replication organelle
Science, Aug 6, 2020; DOI: 10.1126/science.abd3629
Measurement of thickness of the inner mucus layer
Authors: Takahide Ara, Daigo Hashimoto and Takanori Teshima
Date: Feb 24, 2021，Views：1038
Original protocol used in research article：
Intestinal goblet cells protect against GVHD after allogeneic stem cell transplantation via Lypd8
Science Translational Medicine, Jun 30, 2020; DOI: 10.1126/scitranslmed.aaw0720
Task implementations
Author: Jin Fan
Date: Feb 24, 2021，Views：1091
Original protocol used in research article：
Anterior insular cortex plays a critical role in interoceptive attention
eLife, Apr 15, 2019; DOI: 10.7554/eLife.42265
Detection of proteins in membrane fractions
Authors: Alam García-Heredia, Takehiro Kado, Caralyn E. Sein, Julia Puffal, Sarah H. Osman, Julius Judd, Todd A. Gray, Yasu S. Morita and M. Sloan Siegrist
Date: Feb 23, 2021，Views：1087
Original protocol used in research article：
Membrane-partitioned cell wall synthesis in mycobacteria
eLife, Feb 5, 2021; DOI: 10.7554/eLife.60263
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