Protocols shared by authors via RaP service
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MSA-2 formulation for in vivo dosing
Author: Samanthi A. Perera
Date: Jan 11, 2021，Views：983
Original protocol used in research article：
An orally available non-nucleotide STING agonist with antitumor activity
Science, Aug 20, 2020; DOI: 10.1126/science.aba6098
Culture and treatment of BMDMs
Author: Wang Yi
Date: Jan 11, 2021，Views：1042
Original protocol used in research article：
MBD2 serves as a viable target against pulmonary fibrosis by inhibiting macrophage M2 program
Science Advances, Jan 1, 2021; DOI: 10.1126/sciadv.abb6075
Purification of mitoribosomes with Oxa1L from HEK cell
Authors: Hanting Yang and Nirupa Desai
Date: Jan 10, 2021，Views：1204
Original protocol used in research article：
Elongational stalling activates mitoribosome-associated quality control
Science, Nov 26, 2020; DOI: 10.1126/science.abc7782
In vitro Anti-tumor TIL expansion experiment
Author: Sri Krishna
Date: Jan 8, 2021，Views：1074
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
Illumina sequencing and SNP calling
Author: Qingyun Liu
Date: Jan 7, 2021，Views：842
Original protocol used in research article：
Mycobacterium tuberculosis clinical isolates carry mutational signatures of host immune environments
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba4901
PG9 chimeric light chain IgG expression
Author: Roberta P Fuller
Date: Jan 7, 2021，Views：1024
Original protocol used in research article：
Reprogramming the antigen specificity of B cells using genome-editing technologies
eLife, Jan 17, 2019; DOI: 10.7554/eLife.42995
Measurement of CD8+ T cell cytotoxicity
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Jan 6, 2021，Views：867
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Haemocytometry
Authors: Joachim Linssen and Andre J van der Ven
Date: Jan 5, 2021，Views：1106
Original protocol used in research article：
A novel haemocytometric COVID-19 prognostic score developed and validated in an observational multicentre European hospital-based study
eLife, Nov 26, 2020; DOI: 10.7554/eLife.63195
Generation of FAM150A-conditioned and FAM150B-conditioned media
Author: Jikui Guan
Date: Jan 5, 2021，Views：915
Original protocol used in research article：
FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase
eLife, Sep 29, 2015; DOI: 10.7554/eLife.09811
Shelf life testing of antibody-functionalized microchips
Author: Hadi Shafiee
Date: Jan 4, 2021，Views：923
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
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