Bio-101 - Improve Research Reproducibility

Differential analysis of N-glycopeptide abundance and N-glycosylation site occupancy for studying protein N-glycosylation dysregulation in human disease

Authors: Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin
Date: Dec 30, 2020，Views：1091

Original protocol used in research article：

Integrative glycoproteomics reveals protein N-glycosylation aberrations and glycoproteomic network alterations in Alzheimer’s disease
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abc5802

RNA-seq expression analysis

Authors: Stefan Bohn and Nevan J. Krogan
Date: Dec 30, 2020，Views：1348

Original protocol used in research article：

Genetic interaction mapping informs integrative structure determination of protein complexes
Science, Dec 11, 2020; DOI: 10.1126/science.aaz4910

Generation of TILs

Author: Sri Krishna
Date: Dec 29, 2020，Views：1045

Original protocol used in research article：

Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847

Restriction enzyme accessibility assay

Author: Sharon E Torigoe
Date: Dec 29, 2020，Views：2046

Original protocol used in research article：

ATP-dependent chromatin assembly is functionally distinct from chromatin remodeling
eLife, Aug 20, 2013; DOI: 10.7554/eLife.00863

Preparation of single nuclei suspension from adult Drosophila abdominal cuticle

Authors: Arpan C Ghosh and Norbert Perrimon
Date: Dec 28, 2020，Views：1161

Original protocol used in research article：

Drosophila PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.56969

In vitro calcium imaging

Authors: Qiping Dong and Qiang Chang
Date: Dec 28, 2020，Views：1752

Original protocol used in research article：

Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes
eLife, Mar 29, 2018; DOI: 10.7554/eLife.33417

Constructs and stable cell lines

Author: Naeha Subramanian
Date: Dec 28, 2020，Views：997

Original protocol used in research article：

A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses
Science Signaling, Dec 7, 2020; DOI: 10.1126/scisignal.aba3244

PBMC processing and stimulations

Authors: Thanaphong Phongpreecha, Rosemary Fernandez and Thomas Montine
Date: Dec 24, 2020，Views：1078

Original protocol used in research article：

Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575

Lateral hypothalamus surgery

Author: Yeka Aponte
Date: Dec 22, 2020，Views：949

Original protocol used in research article：

High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo
eLife, Jan 4, 2019; DOI: 10.7554/eLife.40805

In vitro cleavage assay and Electrophoretic Mobility Shift Assay (EMSA)

Author: Roger S. Zou
Date: Dec 22, 2020，Views：1116

Original protocol used in research article：

Very fast CRISPR on demand
Science, Jun 11, 2020; DOI: 10.1126/science.aay8204

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