Protocols shared by authors via RaP service
Journal
16S rRNA gene analysis
Author: Xiaoyuan Zhou
Date: Dec 9, 2020，Views：878
Original protocol used in research article：
Gut microbiota–specific IgA+ B cells traffic to the CNS in active multiple sclerosis
Science Immunology, Nov 19, 2020; DOI: 10.1126/sciimmunol.abc7191
LNP preparation
Authors: Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2232
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
LNP transfection
Authors: Dan Peer, Anna Gutkin and Daniel Rosenblum
Date: Dec 8, 2020，Views：2637
Original protocol used in research article：
CRISPR-Cas9 genome editing using targeted lipid nanoparticles for cancer therapy
Science Advances, Nov 18, 2020; DOI: 10.1126/sciadv.abc9450
Phenotyping of adoptively transferred and endogenous tumor-infiltrating CD8+ T cells in the B16F10 orthotopic melanoma model
Authors: Meixi Hao, Caoyun Ju and Can Zhang
Date: Dec 8, 2020，Views：891
Original protocol used in research article：
Combination of metabolic intervention and T cell therapy enhances solid tumor immunotherapy
Science Translational Medicine, Nov 24, 2020; DOI: 10.1126/scitranslmed.aaz6667
Flow cytometry dissociation protocol for Individual Drosophila brains
Author: Shyama Nandakumar
Date: Dec 7, 2020，Views：1045
Original protocol used in research article：
Polyploidy in the adult Drosophila brain
eLife, Aug 25, 2020; DOI: 10.7554/eLife.54385
Evaluating T cell escape in epitopes previously defined as reactive in clade B consensus mapping - Protocol for Elispot, Peptide handling
Authors: Joanna A Warren and Nilu Goonetilleke
Date: Dec 7, 2020，Views：1010
Original protocol used in research article：
The HIV-1 latent reservoir is largely sensitive to circulating T cells
eLife, Oct 6, 2020; DOI: 10.7554/eLife.57246
Transfection, pool maintenance, and drug addition
Authors: Raghuvir Viswanatha and Norbert Perrimon
Date: Dec 7, 2020，Views：968
Original protocol used in research article：
Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells
eLife, Jul 27, 2018; DOI: 10.7554/eLife.36333
Stimuli
Author: Paolo Ossola
Date: Dec 7, 2020，Views：913
Original protocol used in research article：
Belief updating in bipolar disorder predicts time of recurrence
eLife, Nov 10, 2020; DOI: 10.7554/eLife.58891
Single-cell RNA-seq analysis of public 10x data
Authors: Jean-Baptiste Gorin, Vincent Wu, Son Nguyen and Marcus Buggert
Date: Dec 7, 2020，Views：840
Original protocol used in research article：
TOX is expressed by exhausted and polyfunctional human effector memory CD8+ T cells
Science Immunology, Jul 2, 2020; DOI: 10.1126/sciimmunol.aba7918
Isolation of single cells from cryoinjured hearts
Author: Jeroen Bakkers
Date: Dec 5, 2020，Views：897
Original protocol used in research article：
Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart
eLife, Dec 23, 2019; DOI: 10.7554/eLife.50163
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