Protocols shared by authors via RaP service
Journal
Fc.IL-2 muteins
Authors: Daniel J. Campbell and Marc A. Gavin
Date: Feb 1, 2021，Views：967
Original protocol used in research article：
An IL-2 mutein engineered to promote expansion of regulatory T cells arrests ongoing autoimmunity in mice
Science Immunology, Aug 13, 2020; DOI: 10.1126/sciimmunol.aba5264
Ion transport activity assay of microbial rhodopsin expressed in E. coli cells
Authors: Masae Konno, Keiichi Inoue and Hideki Kandori
Date: Jan 31, 2021，Views：5159
Original protocol used in research article：
Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps
Science Advances, Apr 9, 2020; DOI: 10.1126/sciadv.aaz2441
Immunofluorescence staining of heart frozen sections
Author: Huang-Tian Yang
Date: Jan 31, 2021，Views：1177
Original protocol used in research article：
Strontium ions protect hearts against myocardial ischemia/reperfusion injury
Science Advances, Jan 15, 2021; DOI: 10.1126/sciadv.abe0726
ATAC-seq significance analysis
Author: Wen Zhang
Date: Jan 31, 2021，Views：966
Original protocol used in research article：
Chromatin accessibility analysis reveals regulatory dynamics of developing human retina and hiPSC-derived retinal organoids
Science Advances, Feb 6, 2020; DOI: 10.1126/sciadv.aay5247
Western blotting
Authors: Jie Yang and Yuan Wang
Date: Jan 30, 2021，Views：936
Original protocol used in research article：
Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation leading to impaired CD8+ T cell response in tumor
Science Advances, May 28, 2020; DOI: 10.1126/sciadv.aba5412
HCR fluorescent in situ hybridization (FISH) on slides
Author: Natasha M O'Brown
Date: Jan 29, 2021，Views：1453
Original protocol used in research article：
Suppression of transcytosis regulates zebrafish blood-brain barrier function
eLife, Aug 20, 2019; DOI: 10.7554/eLife.47326
Whole-mount tail skin staining and imaging
Authors: Zijian Xu and Ting Chen
Date: Jan 29, 2021，Views：1293
Original protocol used in research article：
Embryonic attenuated Wnt/β-catenin signaling defines niche location and long-term stem cell fate in hair follicle
eLife, Dec 14, 2015; DOI: 10.7554/eLife.10567
Image analysis
Author: Ludovico Cantuti-Castelvetri
Date: Jan 28, 2021，Views：967
Original protocol used in research article：
Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity
Science, Nov 13, 2020; DOI: 10.1126/science.abd2985
Matrigel Invasion Assay Protocol
Authors: Chen Dong, Pooja Popli, Ramakrishna Kommagani and Thorold W Theunissen
Date: Jan 28, 2021，Views：8671
Original protocol used in research article：
Derivation of trophoblast stem cells from naïve human pluripotent stem cells
eLife, Feb 12, 2020; DOI: 10.7554/eLife.52504
X-ray crystallographic analysis of the WT erα LBD-BZA complex
Author: Sean W Fanning
Date: Jan 28, 2021，Views：854
Original protocol used in research article：
The SERM/SERD bazedoxifene disrupts ESR1 helix 12 to overcome acquired hormone resistance in breast cancer cells
eLife, Nov 29, 2018; DOI: 10.7554/eLife.37161
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