Bio-101 - Improve Research Reproducibility

Diffusion-weighted MRI protocol for acute brain edema detection after middle cerebral artery occlusion in mice

Authors: Humberto Mestre, Ting Du, Maiken Nedergaard and Yuki Mori
Date: Jan 15, 2021，Views：4168

Original protocol used in research article：

Cerebrospinal fluid influx drives acute ischemic tissue swelling
Science, Mar 12, 2020; DOI: 10.1126/science.aax7171

CUT&RUN protocol for egg chambers

Authors: Elizabeth M Knapp, Wei Li and Jianjun Sun
Date: Jan 15, 2021，Views：1106

Original protocol used in research article：

Nuclear receptor Ftz-f1 promotes follicle maturation and ovulation partly via bHLH/PAS transcription factor Sim
eLife, Apr 27, 2020; DOI: 10.7554/eLife.54568

Metagenome annotation with dbCAN and AntiSMASH

Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：948

Original protocol used in research article：

Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285

Soil collection, plant bioassays and endosphere sampling

Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：1034

Original protocol used in research article：

Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285

HCR on Whole Mouse Embryos

Authors: Matthew J Anderson and Mark Lewandoski
Date: Jan 13, 2021，Views：1096

Original protocol used in research article：

Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608

CRISPR constructs

Author: Kui Li
Date: Jan 12, 2021，Views：955

Original protocol used in research article：

CD163 and pAPN double-knockout pigs are resistant to PRRSV and TGEV and exhibit decreased susceptibility to PDCoV while maintaining normal production performance
eLife, Sep 2, 2020; DOI: 10.7554/eLife.57132

Immunofluorescence

Author: Shanshan Gao
Date: Jan 12, 2021，Views：810

Original protocol used in research article：

TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy
Science Advances, Jul 9, 2020; DOI: 10.1126/sciadv.aba7822

Study design

Author: Hubert H. Lim
Date: Jan 11, 2021，Views：889

Original protocol used in research article：

Bimodal neuromodulation combining sound and tongue stimulation reduces tinnitus symptoms in a large randomized clinical study
Science Translational Medicine, Oct 6, 2020; DOI: 10.1126/scitranslmed.abb2830

Search for PIN family members from different plant species

Authors: Yuzhou Zhang and Lanxin Li
Date: Jan 11, 2021，Views：859

Original protocol used in research article：

Functional innovations of PIN auxin transporters mark crucial evolutionary transitions during rise of flowering plants
Science Advances, Dec 11, 2020; DOI: 10.1126/sciadv.abc8895

Retrograde DiI labeling

Authors: William Olson and Wenqin Luo
Date: Jan 11, 2021，Views：1655

Original protocol used in research article：

Sparse genetic tracing reveals regionally specific functional organization of mammalian nociceptors
eLife, Oct 12, 2017; DOI: 10.7554/eLife.29507

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