Protocols shared by authors via RaP service
Journal
Generation of FAM150A-conditioned and FAM150B-conditioned media
Author: Jikui Guan
Date: Jan 5, 2021，Views：898
Original protocol used in research article：
FAM150A and FAM150B are activating ligands for anaplastic lymphoma kinase
eLife, Sep 29, 2015; DOI: 10.7554/eLife.09811
Shelf life testing of antibody-functionalized microchips
Author: Hadi Shafiee
Date: Jan 4, 2021，Views：914
Original protocol used in research article：
Virus detection using nanoparticles and deep neural network–enabled smartphone system
Science Advances, Dec 16, 2020; DOI: 10.1126/sciadv.abd5354
In silico SETD2 methylation site analysis
Authors: Riyad N. H. Seervai and Cheryl Lyn Walker
Date: Jan 1, 2021，Views：839
Original protocol used in research article：
The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abb7854
Electromagnetic simulation
Authors: Jahyun Koo and Xie Zhaoqian
Date: Jan 1, 2021，Views：775
Original protocol used in research article：
Wirelessly controlled bioresorbable drug delivery device with active valves that exploit electrochemically triggered crevice corrosion
Science Advances, Aug 27, 2020; DOI: 10.1126/sciadv.abb1093
Differential analysis of N-glycopeptide abundance and N-glycosylation site occupancy for studying protein N-glycosylation dysregulation in human disease
Authors: Qi Zhang, Cheng Ma, Lian Li and Lih-Shen Chin
Date: Dec 30, 2020，Views：1078
Original protocol used in research article：
Integrative glycoproteomics reveals protein N-glycosylation aberrations and glycoproteomic network alterations in Alzheimer’s disease
Science Advances, Oct 1, 2020; DOI: 10.1126/sciadv.abc5802
RNA-seq expression analysis
Authors: Stefan Bohn and Nevan J. Krogan
Date: Dec 30, 2020，Views：1323
Original protocol used in research article：
Genetic interaction mapping informs integrative structure determination of protein complexes
Science, Dec 11, 2020; DOI: 10.1126/science.aaz4910
Generation of TILs
Author: Sri Krishna
Date: Dec 29, 2020，Views：1024
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
Restriction enzyme accessibility assay
Author: Sharon E Torigoe
Date: Dec 29, 2020，Views：1998
Original protocol used in research article：
ATP-dependent chromatin assembly is functionally distinct from chromatin remodeling
eLife, Aug 20, 2013; DOI: 10.7554/eLife.00863
Preparation of single nuclei suspension from adult Drosophila abdominal cuticle
Authors: Arpan C Ghosh and Norbert Perrimon
Date: Dec 28, 2020，Views：1140
Original protocol used in research article：
Drosophila PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.56969
In vitro calcium imaging
Authors: Qiping Dong and Qiang Chang
Date: Dec 28, 2020，Views：1713
Original protocol used in research article：
Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes
eLife, Mar 29, 2018; DOI: 10.7554/eLife.33417
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