Protocols shared by authors via RaP service
Journal
Restriction enzyme accessibility assay
Author: Sharon E Torigoe
Date: Dec 29, 2020，Views：1918
Original protocol used in research article：
ATP-dependent chromatin assembly is functionally distinct from chromatin remodeling
eLife, Aug 20, 2013; DOI: 10.7554/eLife.00863
Preparation of single nuclei suspension from adult Drosophila abdominal cuticle
Authors: Arpan C Ghosh and Norbert Perrimon
Date: Dec 28, 2020，Views：1103
Original protocol used in research article：
Drosophila PDGF/VEGF signaling from muscles to hepatocyte-like cells protects against obesity
eLife, Oct 27, 2020; DOI: 10.7554/eLife.56969
In vitro calcium imaging
Authors: Qiping Dong and Qiang Chang
Date: Dec 28, 2020，Views：1667
Original protocol used in research article：
Mechanism and consequence of abnormal calcium homeostasis in Rett syndrome astrocytes
eLife, Mar 29, 2018; DOI: 10.7554/eLife.33417
Constructs and stable cell lines
Author: Naeha Subramanian
Date: Dec 28, 2020，Views：955
Original protocol used in research article：
A small sustained increase in NOD1 abundance promotes ligand-independent inflammatory and oncogene transcriptional responses
Science Signaling, Dec 7, 2020; DOI: 10.1126/scisignal.aba3244
PBMC processing and stimulations
Authors: Thanaphong Phongpreecha, Rosemary Fernandez and Thomas Montine
Date: Dec 24, 2020，Views：1037
Original protocol used in research article：
Single-cell peripheral immunoprofiling of Alzheimer’s and Parkinson’s diseases
Science Advances, Nov 25, 2020; DOI: 10.1126/sciadv.abd5575
Lateral hypothalamus surgery
Author: Yeka Aponte
Date: Dec 22, 2020，Views：917
Original protocol used in research article：
High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo
eLife, Jan 4, 2019; DOI: 10.7554/eLife.40805
In vitro cleavage assay and Electrophoretic Mobility Shift Assay (EMSA)
Author: Roger S. Zou
Date: Dec 22, 2020，Views：1025
Original protocol used in research article：
Very fast CRISPR on demand
Science, Jun 11, 2020; DOI: 10.1126/science.aay8204
TGIRT-seq library construction
Author: Alan M Lambowitz
Date: Dec 22, 2020，Views：1153
Original protocol used in research article：
Identification of protein-protected mRNA fragments and structured excised intron RNAs in human plasma by TGIRT-seq peak calling
eLife, Sep 2, 2020; DOI: 10.7554/eLife.60743
ATAC sequencing protocol starting from frozen cells
Authors: Monika Mehta and Juan Manuel Caravaca-Guasch
Date: Dec 22, 2020，Views：925
Original protocol used in research article：
Stem-like CD8 T cells mediate response of adoptive cell immunotherapy against human cancer
Science, Dec 11, 2020; DOI: 10.1126/science.abb9847
in vitro Fluorescence Resonance Energy Transfer (FRET) and FRET in cells
Authors: Seunghan Son and Chul Geun Kim
Date: Dec 22, 2020，Views：791
Original protocol used in research article：
Rational discovery of antimetastatic agents targeting the intrinsically disordered region of MBD2
Science Advances, Nov 19, 2019; DOI: 10.1126/sciadv.aav9810
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