Protocols shared by authors via RaP service
Journal
Cell culture
Authors: Manuel Kaulich and Steven F Dowdy
Date: Jan 15, 2021，Views：1155
Original protocol used in research article：
Cyclin D activates the Rb tumor suppressor by mono-phosphorylation
eLife, Jun 4, 2014; DOI: 10.7554/eLife.02872
Behavioral assays
Authors: Charles A Hoeffer and Helen Wong
Date: Jan 15, 2021，Views：940
Original protocol used in research article：
Isoform-specific roles for AKT in affective behavior, spatial memory, and extinction related to psychiatric disorders
eLife, Dec 16, 2020; DOI: 10.7554/eLife.56630
Assays of tagmentation activity of Tn5 on RNA/DNA hybrids
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1102
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
Tn5 in vitro tagmentation on RNA/DNA hybrids
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1283
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
TRACE-seq library preparation and sequencing
Authors: Bo Lu, Liting Dong, Danyang Yi, Meiling Zhang, Chenxu Zhu, Xiaoyu Li and Chengqi Yi
Date: Jan 15, 2021，Views：1207
Original protocol used in research article：
Transposase-assisted tagmentation of RNA/DNA hybrid duplexes
eLife, Jul 23, 2020; DOI: 10.7554/eLife.54919
Diffusion-weighted MRI protocol for acute brain edema detection after middle cerebral artery occlusion in mice
Authors: Humberto Mestre, Ting Du, Maiken Nedergaard and Yuki Mori
Date: Jan 15, 2021，Views：4097
Original protocol used in research article：
Cerebrospinal fluid influx drives acute ischemic tissue swelling
Science, Mar 12, 2020; DOI: 10.1126/science.aax7171
CUT&RUN protocol for egg chambers
Authors: Elizabeth M Knapp, Wei Li and Jianjun Sun
Date: Jan 15, 2021，Views：1059
Original protocol used in research article：
Nuclear receptor Ftz-f1 promotes follicle maturation and ovulation partly via bHLH/PAS transcription factor Sim
eLife, Apr 27, 2020; DOI: 10.7554/eLife.54568
Metagenome annotation with dbCAN and AntiSMASH
Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：927
Original protocol used in research article：
Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285
Soil collection, plant bioassays and endosphere sampling
Author: Víctor J. Carrión
Date: Jan 15, 2021，Views：1012
Original protocol used in research article：
Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome
Science, Nov 1, 2019; DOI: 10.1126/science.aaw9285
HCR on Whole Mouse Embryos
Authors: Matthew J Anderson and Mark Lewandoski
Date: Jan 13, 2021，Views：987
Original protocol used in research article：
Fgf4 maintains Hes7 levels critical for normal somite segmentation clock function
eLife, Nov 19, 2020; DOI: 10.7554/eLife.55608
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